2023 年尼泊尔东部爆发登革热疫情期间登革热病毒的流行血清型和基因型

IF 4 3区 医学 Q2 VIROLOGY
Niten Bharati , Shyam Prakash Dumre , Yogendra Shah , Takeshi Nabesima , Meghnath Dhimal , Srijana Pandey , Merveille Kapandji , Yuki Takamatsu , Takeshi Urano , Basu Dev Pandey , Kouichi Morita , Mya Myat Ngwe Tun , Kishor Pandey
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引用次数: 0

摘要

登革热病毒(DENV)是尼泊尔最主要的蚊媒疾病之一。2023 年,登革热病毒在靠近印度边境的尼泊尔东部开始爆发,并迅速蔓延至全国。本研究旨在描述疫情的流行病学模式、实验室特征、DENV 血清型和基因型。2023 年,在尼泊尔东部贾帕的四家医院开展了一项基于医院的横断面研究。研究人员从登革热疑似患者身上采集了发病 7 天内的急性血清样本,并对样本进行了病毒分离、传统和实时聚合酶链反应(RT-PCR)以及系统发育分析。在 60 份样本中,原发性、继发性和非登革热感染样本分别为 42 份(70%)、11 份(18.3%)和 7 份(11.7%)。在 53 名登革热确诊患者中,46 人(86.7%)对 NS1 呈阳性,12 人(22.6%)对 NS1 和 IgM 均呈阳性。在 42 个登革热分离株中,DENV-2 的世界性基因型新支系最为流行(28 个,66.7%),其次是 DENV-3 的基因型 III(11 个,26.2%)和 DENV-1 的基因型 V(3 个,7.1%)。DENV-3 基因型 III 于 2022-2023 年首次在尼泊尔出现。E基因的系统进化分析表明,尼泊尔的DENV-2分离株与印度和孟加拉国的菌株具有98%的同源核苷酸相似性。据我们所知,这是有关贾帕地区流行的 DENV 血清型和基因型的首次报告。将分子研究结果纳入登革热控制计划可加强监测工作、监测疾病趋势并实施积极措施,以减轻登革热的负担并防止在未来爆发时造成死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Circulating serotypes and genotypes of dengue virus during the 2023 outbreak in Eastern Nepal

Dengue virus (DENV) is one of the most significant mosquito-borne diseases in Nepal. In 2023, DENV outbreaks began in Eastern Nepal, near the border with India, and rapidly spread nationwide. The study aims to describe the outbreak's epidemiological pattern, laboratory characteristics, DENV serotypes, and genotypes. A hospital-based cross-sectional study was conducted in four hospitals in Jhapa, Eastern Nepal, in 2023. Acute serum samples were obtained from dengue suspected patients within 7 days of illness and subjected to virus isolation, conventional and real-time polymerase chain reaction (RT-PCR), and phylogenetic analysis. Out of 60 samples, 42 (70 %), 11 (18.3 %) and 7 (11.7 %) were primary, secondary and non-dengue infection, respectively. Among 53 dengue confirmed patients, 46 (86.7 %) were positive for NS1 and 12 (22.6 %) were positive for both NS1 and IgM. Out of 42 dengue isolates, a new clade of the cosmopolitan genotype of DENV-2 was the most prevalent (28, 66.7 %), followed by genotype III of DENV-3 (11, 26.2 %) and genotype V of DENV-1 (3, 7.1 %). Genotype III of DENV-3 was first introduced in 2022–2023 in Nepal. Phylogenetic analysis of the E gene revealed the DENV-2 isolates from Nepal had 98 % homologous nucleotide similarity with the strains from India and Bangladesh. To our knowledge, this is the first report of circulating serotypes and genotypes of DENV in Jhapa. Integrating molecular findings into the dengue control plan can enhance surveillance efforts, monitor disease trends, and implement proactive measures to reduce the burden of dengue and prevent fatalities in future outbreaks.

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来源期刊
Journal of Clinical Virology
Journal of Clinical Virology 医学-病毒学
CiteScore
22.70
自引率
1.10%
发文量
149
审稿时长
24 days
期刊介绍: The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)
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