研究负载纳米羟基磷灰石和辛伐他汀的三维明胶-纳米纤维素复合支架的体外生物相容性、生物降解性、细胞毒性和分化潜力

IF 2.7 4区 生物学 Q1 ANATOMY & MORPHOLOGY
Soroush Mohammadi , Fahimeh Ghasemi , Seyyedeh Ameneh Alavi G. , Esmat Alemzadeh
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引用次数: 0

摘要

骨组织工程被认为是一种很有前途的骨折愈合解决方案。骨组织工程的一个重要方面是参与骨组织再生和修复的可植入支架。本研究采用冷冻干燥法制作了明胶-纳米纤维素负载纳米羟基磷灰石和辛伐他汀(作为骨诱导成分)的复合支架。从形态、机械、生物降解性、吸水能力和辛伐他汀释放特性等方面对支架进行了表征。此外,还分别使用 MTT 试验和茜素红染色法评估了支架在人骨髓间充质干细胞上的生物相容性和分化潜力。负载辛伐他汀的支架在体外显示出持续释放的特性,最长可达 216 小时。茜素红染色法检测的 BMSCs 分化结果显示,辛伐他汀负载组与其他组之间存在显著差异。此外,MTT 检测结果证实了支架的细胞相容性和无毒性。因此,负载羟基磷灰石和辛伐他汀的明胶-纳米纤维素复合支架有望用于骨组织工程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigate the in vitro biocompatibility, biodegradation, cytotoxicity, and differentiation potential of 3-D gelatin-nanocellulose composite scaffolds loaded with nanohydroxyapatite and simvastatin

Bone tissue engineering has been proposed as a promising solution for healing of bone fractures. An important aspect of bone tissue engineering is the implantable scaffolds that participate in the regeneration and repair of bone tissue. In this study, the composite scaffolds of gelatin- nanocellulose loaded with nanohydroxyapatite and simvastatin (as the osteoinductive component) were fabricated using freeze- drying method. Scaffolds were characterized in terms of morphology, mechanical, biodegradability, water absorption capacity, and simvastatin release characteristics. Also, the biocompatibility and differentiation potential of the scaffolds were evaluated on human bone marrow-derived mesenchymal stem cells using the MTT assay and alizarin red staining, respectively. The simvastatin loaded scaffolds showed a sustained release profile in vitro up to 216 h. The results of BMSCs differentiation by alizarin red staining showed significant differences between the simvastatin loaded group and other groups. Moreover, the results of MTT assay verified cytocompatibility and non-toxicity of the scaffolds. Therefore, the gelatin-nano cellulose composite scaffolds loaded with hydroxyapatite and simvastatin may be considered promising for use in bone tissue engineering.

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来源期刊
Tissue & cell
Tissue & cell 医学-解剖学与形态学
CiteScore
3.90
自引率
0.00%
发文量
234
期刊介绍: Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.
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