Zhiqiang Hu, Yang Li, Bingzhou Zhang, Ying Zhao, Ran Guan, Yapeng Zhou, Jiafa Du, Zhimin Zhang, Xiaowen Li
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This study aimed to analyze serum and colostrum samples from 75 parturient sows with a specific vaccination strategy to measure levels of IgG, IgA, and neutralizing antibodies (nAbs) against PEDV, and to investigate the correlation between serum and colostrum antibody levels, as well as to identify potential biomarkers that can be used to evaluate immunization effects under field conditions.</p><p><strong>Results: </strong>The findings of correlation analysis between antibody levels of IgA, IgG, and nAbs in serum or colostrum samples revealed that IgG demonstrated the most robust correlation with nAbs exhibiting a correlation coefficient of 0.64 in serum samples. Conversely, IgA exhibited the highest correlation with nAbs, with a correlation coefficient of 0.47 in colostrum samples. Additionally, the correlation analysis of antibody levels between serum and colostrum samples indicated that serum IgA displayed the strongest correlation with colostrum IgA, with a coefficient of 0.63, indicating that serum IgA may serve as a viable alternative indicator for evaluating IgA levels in colostrum samples. To further evaluate the suitability of serum IgA as a substitute marker for colostrum IgA, levels of IgA antibodies in serum samples from sows were examined both pre- and post-parturition. The findings indicated that serum IgA levels were initially low prior to the initial immunization, experienced a notable rise 21 days after immunization, and maintained a significant elevation compared to pre-immunization levels from 21 days pre-parturition to 14 days postpartum, spanning a total of 35 days.</p><p><strong>Conclusions: </strong>Serum anti-PEDV IgA antibody levels may serve as a valuable predictor for immunization effects, allowing for the assessment of colostrum IgA antibody levels up to 21 days in advance. This insight could enable veterinarians to timely adjust or optimize immunization strategies prior to parturition, thereby ensuring adequate passive immunity is conferred to piglets through colostral transfer postpartum.</p>","PeriodicalId":20352,"journal":{"name":"Porcine Health Management","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11373460/pdf/","citationCount":"0","resultStr":"{\"title\":\"Serum IgA antibody level against porcine epidemic diarrhea virus is a potential pre-evaluation indicator of immunization effects in sows during parturition under field conditions.\",\"authors\":\"Zhiqiang Hu, Yang Li, Bingzhou Zhang, Ying Zhao, Ran Guan, Yapeng Zhou, Jiafa Du, Zhimin Zhang, Xiaowen Li\",\"doi\":\"10.1186/s40813-024-00382-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Porcine Epidemic Diarrhea (PED) is a highly contagious disease caused by Porcine Epidemic Diarrhea Virus (PEDV), resulting in a mortality rate of suckling piglets as high as 100%. Vaccination is the primary strategy for controlling PEDV infection, however, there is currently a lack of reliable methods for assessing the efficacy of vaccination. This study aimed to analyze serum and colostrum samples from 75 parturient sows with a specific vaccination strategy to measure levels of IgG, IgA, and neutralizing antibodies (nAbs) against PEDV, and to investigate the correlation between serum and colostrum antibody levels, as well as to identify potential biomarkers that can be used to evaluate immunization effects under field conditions.</p><p><strong>Results: </strong>The findings of correlation analysis between antibody levels of IgA, IgG, and nAbs in serum or colostrum samples revealed that IgG demonstrated the most robust correlation with nAbs exhibiting a correlation coefficient of 0.64 in serum samples. Conversely, IgA exhibited the highest correlation with nAbs, with a correlation coefficient of 0.47 in colostrum samples. Additionally, the correlation analysis of antibody levels between serum and colostrum samples indicated that serum IgA displayed the strongest correlation with colostrum IgA, with a coefficient of 0.63, indicating that serum IgA may serve as a viable alternative indicator for evaluating IgA levels in colostrum samples. To further evaluate the suitability of serum IgA as a substitute marker for colostrum IgA, levels of IgA antibodies in serum samples from sows were examined both pre- and post-parturition. 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引用次数: 0
摘要
背景:猪流行性腹泻(PED)是由猪流行性腹泻病毒(PEDV)引起的一种高度传染性疾病,导致哺乳仔猪的死亡率高达 100%。接种疫苗是控制 PEDV 感染的主要策略,但目前缺乏可靠的方法来评估疫苗接种的效果。本研究旨在分析 75 头采用特定疫苗接种策略的经产母猪的血清和初乳样本,以测定针对 PEDV 的 IgG、IgA 和中和抗体(nAbs)水平,并研究血清和初乳抗体水平之间的相关性,以及确定可用于评估现场条件下免疫效果的潜在生物标志物:血清或初乳样本中 IgA、IgG 和 nAbs 抗体水平之间的相关性分析结果显示,IgG 与 nAbs 的相关性最强,血清样本中的相关系数为 0.64。相反,IgA 与 nAbs 的相关性最高,在初乳样本中的相关系数为 0.47。此外,对血清和牛初乳样本抗体水平的相关性分析表明,血清 IgA 与牛初乳 IgA 的相关性最强,相关系数为 0.63,这表明血清 IgA 可以作为评估牛初乳样本中 IgA 水平的一个可行的替代指标。为了进一步评估血清 IgA 作为初乳 IgA 替代指标的适用性,对母猪产前和产后血清样本中的 IgA 抗体水平进行了检测。研究结果表明,初次免疫前血清 IgA 水平较低,免疫后 21 天明显上升,从产仔前 21 天到产后 14 天,共 35 天,血清 IgA 水平与免疫前水平相比保持显著升高:结论:血清抗 PEDV IgA 抗体水平可作为免疫效果的重要预测指标,可提前 21 天评估初乳 IgA 抗体水平。这一洞察力可使兽医在产前及时调整或优化免疫策略,从而确保仔猪产后通过初乳转移获得足够的被动免疫。
Serum IgA antibody level against porcine epidemic diarrhea virus is a potential pre-evaluation indicator of immunization effects in sows during parturition under field conditions.
Background: Porcine Epidemic Diarrhea (PED) is a highly contagious disease caused by Porcine Epidemic Diarrhea Virus (PEDV), resulting in a mortality rate of suckling piglets as high as 100%. Vaccination is the primary strategy for controlling PEDV infection, however, there is currently a lack of reliable methods for assessing the efficacy of vaccination. This study aimed to analyze serum and colostrum samples from 75 parturient sows with a specific vaccination strategy to measure levels of IgG, IgA, and neutralizing antibodies (nAbs) against PEDV, and to investigate the correlation between serum and colostrum antibody levels, as well as to identify potential biomarkers that can be used to evaluate immunization effects under field conditions.
Results: The findings of correlation analysis between antibody levels of IgA, IgG, and nAbs in serum or colostrum samples revealed that IgG demonstrated the most robust correlation with nAbs exhibiting a correlation coefficient of 0.64 in serum samples. Conversely, IgA exhibited the highest correlation with nAbs, with a correlation coefficient of 0.47 in colostrum samples. Additionally, the correlation analysis of antibody levels between serum and colostrum samples indicated that serum IgA displayed the strongest correlation with colostrum IgA, with a coefficient of 0.63, indicating that serum IgA may serve as a viable alternative indicator for evaluating IgA levels in colostrum samples. To further evaluate the suitability of serum IgA as a substitute marker for colostrum IgA, levels of IgA antibodies in serum samples from sows were examined both pre- and post-parturition. The findings indicated that serum IgA levels were initially low prior to the initial immunization, experienced a notable rise 21 days after immunization, and maintained a significant elevation compared to pre-immunization levels from 21 days pre-parturition to 14 days postpartum, spanning a total of 35 days.
Conclusions: Serum anti-PEDV IgA antibody levels may serve as a valuable predictor for immunization effects, allowing for the assessment of colostrum IgA antibody levels up to 21 days in advance. This insight could enable veterinarians to timely adjust or optimize immunization strategies prior to parturition, thereby ensuring adequate passive immunity is conferred to piglets through colostral transfer postpartum.
期刊介绍:
Porcine Health Management (PHM) is an open access peer-reviewed journal that aims to publish relevant, novel and revised information regarding all aspects of swine health medicine and production.