间充质干细胞和聚乙烯醇包裹的硒纳米粒子对阿尔茨海默样表型大鼠的影响

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Siamak Shahidi, Sara Soleimani Asl, Bahareh Gholamigeravand, Simin Afshar, Nasrin Hashemi-Firouzi, Alireza Samzadeh-Kermani, Mahsa Majidi, Kimia Amiri
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引用次数: 0

摘要

目的:间充质干细胞(MSC)移植是治疗阿尔茨海默病(AD)的一种很有前景的方法。然而,这些干细胞移植到受体动物体内后寿命很短。硒纳米颗粒因其尺寸小,有助于药物输送治疗脑部疾病。本研究调查了间充质干细胞和聚乙烯醇(PVA)包裹的硒纳米颗粒(SeNPs)在大鼠AD模型中的治疗效果:通过脑室内注射链脲佐菌素(STZ)诱导阿尔茨海默样表型。大鼠被分为五组:对照组、Alz组(STZ;3 mg/kg,10 μl,ICV)、Alz+干细胞组(ICV移植)、Alz+SeNP组(0.4 mg/kg,口服)和Alz+干细胞+SeNPs组。在Alz组中,STZ的ICV给药模拟了AD的某些方面。STZ 给药后,SeNPs 给药 30 天。新物体识别(NOR)和被动回避学习(PAL)测试用于评估认知和记忆能力。氧化应激生物标志物和脑源性神经营养因子(BDNF)分别通过生化分析、ELISA试剂盒和刚果红染色进行评估:结果:与单独使用SeNPs或间叶干细胞相比,PVA包裹的SeNPs和间叶干细胞移植联合疗法在增强记忆再获得方面更为有效。干细胞与PVA包被的SeNPs的联合使用显著提高了抗氧化能力:结果表明,在动物模型中,PVA包被的SeNPs和间充质干细胞的联合治疗对AD具有相当大的神经保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of mesenchymal stem cells and polyvinyl alcohol-coated selenium nanoparticles on rats with Alzheimer-like phenotypes.

Objectives: Mesenchymal stem cell (MSC) transplantation represents a promising approach for treating Alzheimer's disease (AD). These stem cells, however, have a short lifespan following transplantation into recipient animals. Selenium nanoparticles, due to their size, aid in drug delivery for brain disorders. This study investigated the therapeutic effect of MSCs and polyvinyl alcohol (PVA)-coated selenium nanoparticles (SeNPs) in a rat model of AD.

Materials and methods: An Alzheimer-like phenotype was induced through intracerebroventricular (ICV) administration of streptozotocin (STZ). Rats were assigned to five groups: control, Alz (STZ; 3 mg/kg, 10 μl, ICV), Alz+stem cell (ICV transplantation), Alz+SeNP (0.4 mg/kg, orally), and Alz+stem cell+SeNPs. The ICV administration of STZ mimicked some aspects of AD in the Alz groups. SeNPs were administrated for 30 days following STZ administration. The novel object recognition (NOR) and passive avoidance learning (PAL) tests were used to evaluate cognition and memory. Oxidative stress biomarkers and brain-derived neurotrophic factor (BDNF) were assessed by biochemical analysis, ELISA kits, and Congo red staining, respectively.

Results: The combined therapy of PVA-coated SeNPs and MSC transplantation was more effective in enhancing memory reacquisition compared to either SeNPs or MSCs alone. The use of stem cells in conjunction with PVA-coated SeNPs significantly boosted anti-oxidant capacity.

Conclusion: The results suggest that the joint treatment with PVA-coated SeNPs and MSCs offers considerable neuroprotection against AD in animal models.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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