Yangchun Liu, Jialing Zhang, Bingjie Zhang, Xuming Mao, Yiman Wang, Yanhong Wang, Meng Fan, Xuan Liu, Jin An, Hongzhong Jin, Li Li
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引用次数: 0
摘要
背景:大疱性类天疱疮(BP)是一种严重的自身免疫性表皮下大疱性疾病。外泌体是大多数细胞类型分泌的小细胞外囊泡。外泌体膜蛋白与多种生物和病理途径有关。本研究旨在探讨外泌体在牛皮癣病理机制中的潜在作用:研究设计:我们收集了 30 名血压患者和 31 名健康对照者的血浆样本。研究设计:我们采集了 30 名 BP 患者和 31 名健康对照者的血浆样本,采用纳米粒子追踪分析法(NTA)分析外泌体的大小和浓度。免疫金标记实验和细胞外囊泡(EV)阵列检测了外泌体的含量和分布:结果:BP组和对照组血浆中的外泌体均被成功提取。EV 阵列显示,BP 组的 CD63 和 CD9 水平明显高于对照组(p p p p 结论):BP180 自身抗原片段在 BP 患者的外泌体膜上表达。外泌体上的 BP180 ICD 和 CD63 有可能成为监测疾病活动的新型生物标记物。
Isolation and analysis of the exosomal membrane proteins in bullous pemphigoid.
Background: Bullous pemphigoid (BP) is a severe autoimmune sub-epidermal bullous disease. Exosomes are small extracellular vesicles secreted by most cell types. The exosomal membrane proteins are implicated in various biological and pathological pathways. This study aims to explore the potential roles of exosomes in BP pathomechanism.
Research design: We collected plasma samples from 30 BP patients and 31 healthy controls. Nanoparticle tracking analysis (NTA) was used to analyze the size and concentration of exosomes. The immunogold labelling experiment and extracellular vesicle (EV) array were performed to detect the content and distribution of exosomes.
Results: The exosomes from both the BP and control groups' plasma were successfully extracted. EV Array showed that CD63 and CD9 levels were significantly higher in the BP group than in the control group (p < 0.05). Expression levels of the BP180 NC16A and intracellular domain (ICD) were higher in the anti-BP180 positive group versus the controls (p < 0.05). The active BP group exhibits higher CD63 and BP180 ICD protein concentrations than the control or inactive BP groups (p < 0.05).
Conclusion: BP180 autoantigen fragments were expressed on the exosomal membrane in BP patients. The BP180 ICD and CD63 on exosomes could potentially be novel biomarkers for monitoring disease activity.
期刊介绍:
Expert Review of Clinical Immunology (ISSN 1744-666X) provides expert analysis and commentary regarding the performance of new therapeutic and diagnostic modalities in clinical immunology. Members of the International Editorial Advisory Panel of Expert Review of Clinical Immunology are the forefront of their area of expertise. This panel works with our dedicated editorial team to identify the most important and topical review themes and the corresponding expert(s) most appropriate to provide commentary and analysis. All articles are subject to rigorous peer-review, and the finished reviews provide an essential contribution to decision-making in clinical immunology.
Articles focus on the following key areas:
• Therapeutic overviews of specific immunologic disorders highlighting optimal therapy and prospects for new medicines
• Performance and benefits of newly approved therapeutic agents
• New diagnostic approaches
• Screening and patient stratification
• Pharmacoeconomic studies
• New therapeutic indications for existing therapies
• Adverse effects, occurrence and reduction
• Prospects for medicines in late-stage trials approaching regulatory approval
• Novel treatment strategies
• Epidemiological studies
• Commentary and comparison of treatment guidelines
Topics include infection and immunity, inflammation, host defense mechanisms, congenital and acquired immunodeficiencies, anaphylaxis and allergy, systemic immune diseases, organ-specific inflammatory diseases, transplantation immunology, endocrinology and diabetes, cancer immunology, neuroimmunology and hematological diseases.