柠檬汁和果皮成分能有效稳定大鼠腹膜肥大细胞

IF 2.5 Q3 CELL BIOLOGY
Aki Sato, Yu Kikuta, Itsuro Kazama
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引用次数: 0

摘要

背景/目的:柠檬(Citrus limon)含有多种营养物质,是最受欢迎的柑橘类水果之一。除了具有抗氧化、抗癌、抗菌和抗炎特性外,临床研究还表明其具有抗过敏特性:方法:我们使用微分干涉对比(DIC)显微镜研究了柠檬汁和柠檬皮成分(如柠檬酸、抗坏血酸、橙皮素和麦角酚)对大鼠腹腔肥大细胞脱颗粒的影响。我们还利用水溶性染料荧光成像技术研究了它们对质膜变形的影响:结果:柠檬汁剂量依赖性地减少了脱颗粒肥大细胞的数量。在浓度等于或高于 0.25 毫摩尔时,柠檬酸、橙皮甙和二碘酪醇以剂量依赖的方式显著减少脱颗粒肥大细胞的数量,而抗坏血酸则需要更高的剂量才能产生显著效果。在 1 mM 的剂量下,柠檬酸、橙皮素和麦角二酚几乎完全抑制了肥大细胞的外排,并洗去了困在肥大细胞表面的荧光黄,而抗坏血酸则没有:本研究首次在体外证明柠檬酸、橙皮素和二十二烷醇等柠檬成分具有有效的肥大细胞稳定特性。这些特性可归因于它们对脱颗粒肥大细胞质膜变形的抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lemon Juice and Peel Constituents Potently Stabilize Rat Peritoneal Mast Cells.

Background/aims: Lemons (Citrus limon ) contain various nutrients and are among the most popular citrus fruit. Besides their antioxidant, anticancer, antibacterial, and anti-inflammatory properties, clinical studies have indicated their anti-allergic properties.

Methods: Using the differential-interference contrast (DIC) microscopy, we examined the effects of lemon juice and peel constituents, such as citric acid, ascorbic acid, hesperetin and eriodictyol, on the degranulation from rat peritoneal mast cells. Using fluorescence imaging with a water-soluble dye, Lucifer Yellow, we also examined their effects on the deformation of the plasma membrane.

Results: Lemon juice dose-dependently decreased the number of degranulated mast cells. At concentrations equal to or higher than 0.25 mM, citric acid, hesperetin, and eriodictyol significantly reduced the number of degranulating mast cells in a dose-dependent manner, while ascorbic acid required much higher doses to exert significant effects. At 1 mM, citric acid, hesperetin, and eriodictyol almost completely inhibited exocytosis and washed out the Lucifer Yellow trapped on the mast cell surface, while ascorbic acid did not.

Conclusion: This study provides in vitro evidence for the first time that lemon constituents, such as citric acid, hesperetin, and eriodictyol, potently exert mast cell-stabilizing properties. These properties are attributable to their inhibitory effects on plasma membrane deformation in degranulating mast cells.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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