基于 LC-MS 的关键试剂生物素化影响因素综合研究。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2024-01-01 Epub Date: 2024-09-04 DOI:10.1080/17576180.2024.2394317
Wanlu Qu, Jim Glick, Paola Dessanti, Jennifer Cunliffe
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引用次数: 0

摘要

目的:关键试剂(CR)应用于配体结合测定(LBA),而生物素化是一种广泛用于关键试剂的连接方法。然而,表征不充分和生物素化不一致会导致 LBA 失败,需要进行大量的故障排除工作。本论文开发了生物素化 CR 的检测方法,并评估了生物素化条件的效率,以确保试剂的可靠性和在 LBA 中实施时的准确性:应用完整质量分析鉴定具有复杂糖基化和生物素化模式的CR。结果:生物素酰化程度和位点均发生了变化:结果:生物素化程度和位点清晰可见:结论:成功鉴定了 A CR 及其生物素化。结论:成功鉴定了一种 CR 及其生物素化特征,明确了生物素化效率与标记条件之间的关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comprehensive LC-MS based study of factors influencing biotinylation of critical reagents.

Aim: Critical reagents (CR) are applied in ligand binding assays (LBA) and biotinylation is a widely conjugation method used for critical reagents. However, insufficient characterization and inconsistent biotinylation can lead to LBA failures and necessitate extensive troubleshooting. This publication developed the detection of biotinylated CR and evaluates efficiency of biotinylation conditions to ensure the reliability of reagents and accuracy when implemented in LBA.Materials & methods: Intact mass analysis was applied to characterize a CR with complex glycosylation and biotinylation patterns. Peptide mapping was developed to identify the biotinylation sites.Results: Biotinylation degrees and sites were clearly illustrated.Conclusion: A CR and its biotinylation were successfully characterized. The relationship between biotinylation efficiency and labeling conditions was clearly illustrated.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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