低频交变磁场对脂肪分解酵母生产赤藓糖醇的影响。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Hong Wang, Jiayang Hou, Dongxu Wang, Hu Shi, Luqian Gong, Xuemeng Lv, Jinlong Liu
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引用次数: 0

摘要

许多研究报告指出,磁场是能够影响微生物新陈代谢的信号。然而,人们对磁场对模式微生物脂溶性亚罗威氏菌(Y. lipolytica)生产赤藓糖醇的影响知之甚少。因此,我们研究了不同磁场强度(0-1.5 mT)和不同磁场处理时间(1-10 天)的低频交变磁场(LF-AMF)对 Y. lipolytica -JZ204 生产赤藓糖醇的影响。最佳处理条件是 0.5 mT,8 天。结果,赤藓糖醇的最大产量为 63.74 克/升,生物量达到 37 克/升,单位生物量的赤藓糖醇产量为 1.7227 克/克,分别比对照组高出 60.72%、32.09% 和 24.85%。我们利用转录组学和 RT-qPCR 技术研究了磁场影响的内部机制。本研究证明了 LF-AMF 在提高 Y. lipolytica JZ-204 赤藓糖醇产量方面的有效性,为磁场在辅助微生物发酵和提高有益产品合成方面的应用提供了启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effect of low frequency alternating magnetic field for erythritol production in Yarrowia lipolytica

Effect of low frequency alternating magnetic field for erythritol production in Yarrowia lipolytica

Effect of low frequency alternating magnetic field for erythritol production in Yarrowia lipolytica

Numerous works have reported that magnetic fields serve as signals capable of influencing microbial metabolism. However, little is known about the effect of magnetic field on erythritol production by the model microorganism Yarrowia lipolytica (Y. lipolytica). Therefore, we investigated the effect of low-frequency alternating magnetic fields (LF-AMF) with different magnetic field intensities (0–1.5 mT) and different magnetic field treatment times (1–10 days) on the production of erythritol by Y. lipolytica -JZ204. The optimal treatment condition was 0.5 mT for 8 days. As a result, a maximal erythritol yield was achieved 63.74 g/L, the biomass was reached 37 g/L, and the specific erythritol yield per unit of biomass was 1.7227 g/g, which were 60.72%, 32.09%, and 24.85% higher than the control, respectively. We investigated the internal mechanism of magnetic fields impact by using transcriptomics and RT-qPCR technology. This study demonstrated the effectiveness of LF-AMF in enhancing erythritol production by Y. lipolytica JZ-204, providing insights for the application of magnetic field in assisting microbial fermentation and improving the synthesis of beneficial products.

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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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