敲除 Fzr 可通过阻断内循环抑制 Nilaparvata lugens 的生长。

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-09-04 DOI:10.1002/ps.8403

Haoli Gao, Xiaowei Yuan, Jingting Wang, Yangyang Yan, Xinyu Zhang, Tianshun He, Xumin Lin, Huihui Zhang, Zewen Liu

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引用次数: 0

摘要

背景：内循环可产生被称为 "多倍体 "的细胞。Fizzy相关蛋白（Fzr）在有丝分裂到内轮分裂的转变过程中发挥着重要作用。褐飞虱 Nilaparvata lugens (Stål) 是一种严重的害虫，专门以水稻为食。然而，人们对 BPH 的多倍体及其调控机制知之甚少：结果：我们在研究唾液腺 FH 和 AG 的多倍性时发现，腺泡 H（FH）和附属腺体（AG）的多倍性水平随 BPH 年龄的增长而显著增加。Fzr 被鉴定为 BPH 唾液腺多倍体的重要调节因子。敲除 Fzr 会导致若虫唾液腺细胞大小和 DNA 含量下降。敲除Fzr会转录上调细胞周期蛋白依赖性激酶1（CDK1）、CDK2、细胞周期蛋白A（CycA）和CycB，下调CycD、CycE、Myc和迷你染色体维护蛋白2-7（MCM2-7）。从表型上看，Fzr基因敲除明显抑制了BPH若虫唾液蛋白的产生、摄食和存活：我们的研究结果表明，BPH若虫唾液腺表现出明显的多倍体性，Fzr对若虫唾液腺的内循环有积极的调节作用。这些发现为研究昆虫多倍体的调控机制提供了线索。© 2024 化学工业学会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Knockdown of Fzr inhibited the growth of Nilaparvata lugens by blocking endocycle

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Knockdown of Fzr inhibited the growth of Nilaparvata lugens by blocking endocycle

Background

The endocycle can generate cells referred to as ‘polyploid’. Fizzy-related protein (Fzr) plays an important role in driving the mitosis-to-endocycle transition. The brown planthopper (BPH), Nilaparvata lugens (Stål), a serious insect pest, feeds exclusively on rice. However, polyploidy and its regulatory mechanisms are poorly understood in BPH.

Results

Here, we found that the ploidy levels of follicles H (FH) and accessory gland (AG) significantly increased with BPH age when examining the polyploidy of FH and AG of salivary glands. Fzr was identified as an important regulator for polyploidy in BPH salivary gland. Knockdown of Fzr resulted in a decrease in cell size and DNA content in nymph salivary glands. Fzr knockdown transcriptionally upregulated cyclin-dependent kinase 1 (CDK1), CDK2, cyclin A (CycA) and CycB, and downregulated CycD, CycE, Myc and mini-chromosome maintenance protein 2-7 (MCM2-7). Phenotypically, Fzr knockdown significantly suppressed salivary protein production, feeding and survival in BPH nymphs.

Conclusion

Our results show that BPH salivary glands exhibit obvious polyploidy, and Fzr positively regulates the endocycle in nymph salivary gland. These findings provide clues for the study of the regulatory mechanisms of insect polyploidy. © 2024 Society of Chemical Industry.

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.