鸢尾素对多囊卵巢综合征大鼠卵巢磷脂酰肌醇-3-激酶/蛋白激酶B信号通路和丝裂原活化蛋白激酶/细胞外信号调节激酶通路的影响

IF 1.6 4区 医学 Q3 OBSTETRICS & GYNECOLOGY
Cheng-gang Li, Li Zhou, Ying-jun Zhang, Yong Li, Li-yan Zhao
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引用次数: 0

摘要

目的研究鸢尾素对多囊卵巢综合征(PCOS)卵巢胰岛素抵抗(IR)的独立影响,并探讨可能的影响途径:用 Poretsky L'method 建立 PCOS Medel,然后将 PCOS 大鼠随机分为模型组(M)和鸢尾素组(I),正常大鼠(N)作为对照。然后给 I 组大鼠注射重组鸢尾素。然后观察各组大鼠的循环空腹血糖(FBG)、空腹胰岛素(FINS)、体内平衡模型评估(HOMA-IR)、PI3K/AKT和MAPK/ERK通路的水平,以及鸢尾素对多囊卵巢综合征大鼠卵巢循环HOMA-IR、PI3K/AKT和MAPK/ERK通路水平的影响:结果:与正常组相比,模型组的FBG、FINS和HOMA-IR水平均显著升高(P我们的研究表明,鸢尾素不仅能改善循环中的胰岛素抵抗,还能通过提高 PI3K/AKT 信号的活性和降低 MAPK/ERK 信号的活性来改善卵巢 IR。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of irisin on ovarian phosphatidylinositol-3-kinase/protein kinase B signaling pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathways of rats with polycystic ovary syndrome

Objective

To investigate the independent effects of irisin on insulin resistance (IR) in ovary of polycystic ovary syndrome (PCOS) and explore possible pathways.

Methods

We established PCOS medel using Poretsky L's method, then PCOS rats were randomly divided into model group (M) and irisin group (I), and normal rats (N) were used as the control. Then rats in the group I were injected with recombinant irisin. Then the levels of circulating fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of IR (HOMA-IR) and PI3K/AKT and MAPK/ERK pathways in each group were observed, as well as the effects of irisin on the levels of circulating HOMA-IR and PI3K/AKT and MAPK/ERK pathways in ovary of PCOS rats were evaluated.

Results

Compared with normal group, levels of FBG, FINS, and HOMA-IR of model group were significantly increased (p < 0.001, p < 0.001, and p < 0.001, respectively), levels of average optical density by IHC of p-PI3K, PI3K, p-AKT, and AKT (p = 0.015, p = 0.010, p = 0.005, and p = 0.009, respectively) and levels of mRNA concentration of PI3K and AKT (p = 0.001, and p = 0.005, respectively) were decreased, while the levels of average optical density of p-ERK, ERK (p = 0.011, and p = 0.013, respectively) and level of mRNA concentration of ERK (p < 0.001) were increased in ovary. After irisin intervention, compared with model group, levels of FBG, FINS, and HOMA-IR of rats in irisin group were significantly decreased (p = 0.001, p < 0.001, and p < 0.001, respectively), levels of average optical density by IHC of p-PI3K, PI3K, p-AKT, and AKT (p = 0.030, p = 0.024, p = 0.012, and p = 0.025, respectively) and levels of mRNA concentration of PI3K and AKT (p = 0.002, and p = 0.003, respectively) were significantly increased, while the levels of average optical density of p-ERK, ERK (p = 0.004, and p = 0.026, respectively) and level of mRNA concentration of ERK (p = 0.001) were significantly decreased.

Conclusion

Our study demonstrated that irisin could not only improve circulating insulin resistance, but may also improve ovarian IR through an increase in the activity of PI3K/AKT signaling and a decrease of MAPK/ERK signaling.

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来源期刊
CiteScore
3.10
自引率
0.00%
发文量
376
审稿时长
3-6 weeks
期刊介绍: The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.
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