枯草芽孢杆菌在 "会呼吸 "的容器中生长，不使用喷射器通气。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-10-22 DOI:10.1093/bbb/zbae120

Ken-Ichi Yoshida, Kyosuke Yokoyama, Ayşegül Öktem, Shu Ishikawa, Jan Maarten van Dijl, Masato Yotsuya, Ryosuke Sato

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引用次数: 0

摘要

在这里，我们介绍一种由膨化聚四氟乙烯组成的 "呼吸 "容器，它允许气体交换，但不允许液体渗透。内部的细菌培养只需搅拌即可促进空气供应。利用这种装置，用于生产丝胶肌醇的枯草芽孢杆菌细胞工厂得以生长，并高效地生产出丝胶肌醇。结果表明，我们的方法是一种可持续的 "绿色 "细胞工厂方法。

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Bacillus subtilis grown in a "breathing" vessel without sparger aeration.

Here we present a "breathing" vessel consisting of expanded polytetrafluoroethylene, which allows gas exchange but no liquid permeation. The bacterial culture inside needs only agitation to promote air supply. Using this setup, a Bacillus subtilis cell factory for scyllo-inositol production grew to produce scyllo-inositol efficiently. The results indicate that our approach represents a sustainable "greener" approach for the cell factory.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).