三维打印磷酸钙支架可模拟骨骼微观结构,促进骨骼再生和修复。

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Kyohei Takase, Takahiro Niikura, Tomoaki Fukui, Yohei Kumabe, Kenichi Sawauchi, Ryo Yoshikawa, Yuya Yamamoto, Ryota Nishida, Tomoyuki Matsumoto, Ryosuke Kuroda, Keisuke Oe
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引用次数: 0

摘要

三维支架中相互连接的结构允许细胞和营养物质的移动。因此,本研究旨在调查三维打印的β-磷酸三钙(β-TCP)和羟基磷灰石(HAP)支架在体内复制生物骨骼的生物活性。这项研究包括 24 周大的雄性新西兰白兔。在股骨远端外侧创建了一个直径为 4.5 毫米、深度为 8 毫米的圆柱形骨缺损。在右侧股骨(实验侧)植入 3D 打印支架,左侧股骨(对照侧)则不植入支架。术后4周、8周和12周,对骨缺损部位进行了显微CT分析和组织学观察,以跟踪骨修复的进展情况。对照组一侧骨缺损的髓腔内没有发现新骨组织形成的迹象。相反,在实验侧,三维支架表现出了足够的生物活性,导致了新骨组织的生长。随着时间的推移,新骨组织逐渐从外围向中心延伸,这一现象在显微 CT 图像和活检染色中都很明显。在本研究中,我们观察到参与骨代谢的细胞在我们新设计的具有骨微结构的三维打印支架上附着、扩散和增殖。因此,这种支架具有足够的生物活性来诱导新骨形成,有望成为比现有版本更有用的人工骨。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Three-dimensional printed calcium phosphate scaffolds emulate bone microstructure to promote bone regrowth and repair

Three-dimensional printed calcium phosphate scaffolds emulate bone microstructure to promote bone regrowth and repair

The interconnected structures in a 3D scaffold allows the movement of cells and nutrients. Therefore, this study aimed to investigate the in-vivo bioactivity of 3D-printed β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAP) scaffolds that replicate biological bone. This study included 24-week-old male New Zealand white rabbits. A cylindrical bone defect with a diameter of 4.5 mm and a depth of 8 mm was created in the lateral aspect of the distal femur. A 3D-printed scaffold was implanted in the right femur (experimental side), whereas the left femur was kept free of implantation (control side). Micro-CT analysis and histological observations of the bone defect site were conducted at 4, 8, and 12 weeks postoperatively to track the bone repair progress. No evidence of new bone tissue formation was found in the medullary cavity of the bone defect on the control side. In contrast, on the experimental side, the 3D scaffold demonstrated sufficient bioactivity, leading to the growth of new bone tissue. Over time, new bone tissue gradually extended from the periphery toward the center, a phenomenon evident in both micro-CT images and biopsy staining. In the current study, we observed that the cells involved in bone metabolism adhered, spread, and proliferated on our newly designed 3D-printed scaffold with a bone microstructure. Therefore, it is suggested that this scaffold has sufficient bioactivity to induce new bone formation and could be expected to be a more useful artificial bone than the existing version.

Graphical Abstract

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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