{"title":"[紫檀素调节核因子 E2 相关因子 2 对体外结肠癌细胞凋亡的影响]。","authors":"Xue-Hui Shi, Chong-Xi Fan, Quan-Long Yang, Xiao-Ying Wang, Dong-Lin Zhao, Man-Hua Li, Xue-Liang Wu, Jian-Chun Fan, Shou-Bin Ning","doi":"10.3881/j.issn.1000-503X.15988","DOIUrl":null,"url":null,"abstract":"<p><p>Objective To investigate the effects of pterostilbene on human colon cancer LoVo cells and study the regulatory mechanism of nuclear factor E2-related factor 2 (Nrf2) in the process of pterostilbene acting on LoVo cells. Methods LoVo cells were treated with different concentrations (5,10,20,40,60,80,100 μmol/L) of pterostilbene.Cell viability,migration,invasion,and apoptosis were examined by CCK-8,scratch,Transwell,and TUNEL assays,respectively.The mitochondrial membrane potential was measured by the mitochondrial membrane potential assay kit with JC-1.The reactive oxygen species level was measured by 2',7'-dichlorofluorescein diacetate.The protein levels of Nrf2,phosphorylated Nrf2,heme oxygenase 1,and apoptotic proteins (Bcl2 and Bax) were determined by Western blotting.In addition,cell viability,Nrf2 expression,and apoptosis rate were determined after co-application of the Nrf2-specific agonist sulforaphane. Results Compared with the control group,40,60,80,100 μmol/L pterostilbene reduced the viability of LoVo cells (<i>P</i>=0.014,<i>P</i><0.001,<i>P</i><0.001,<i>P</i><0.001).Pterostilbene at 5,10,20 μmol/L did not show effects on cell viability but inhibited cell migration (<i>P</i>=0.008,<i>P</i><0.001,<i>P</i><0.001) and invasion (all <i>P</i><0.001).Pterostilbene at 40,60,80 μmol/L increased apoptosis (<i>P</i>=0.014,<i>P</i><0.001,<i>P</i><0.001),promoted mitochondrial membrane potential depolarization (<i>P</i>=0.026,<i>P</i><0.001,<i>P</i><0.001) and reactive oxygen species accumulation (all <i>P</i><0.001),and down-regulated the expression of phosphorylated Nrf2 (<i>P</i>=0.030,<i>P</i><0.001,<i>P</i><0.001),heme oxygenase 1 (<i>P</i>=0.015,<i>P</i><0.001,<i>P</i><0.001),and Bcl2 (<i>P</i>=0.039,<i>P</i><0.001,<i>P</i><0.001) in LoVo cells.Pterostilbene at 60,80 μmol/L down-regulated Nrf2 expression (<i>P</i>=0.001,<i>P</i><0.001) and up-regulated Bax expression (both <i>P</i><0.001).The application of sulforaphane reversed the effects of pterostilbene on cell viability (<i>P</i><0.001),apoptosis (<i>P</i><0.001),and Nrf2 expression (<i>P</i>=0.022). Conclusion Pterostilbene is a compound that can effectively inhibit colon cancer cells by inhibiting the Nrf2 pathway.</p>","PeriodicalId":6919,"journal":{"name":"中国医学科学院学报","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effect of Pterostilbene Regulating Nuclear Factor E2-Related Factor 2 on Apoptosis of Colon Cancer Cells <i>in Vitro</i>].\",\"authors\":\"Xue-Hui Shi, Chong-Xi Fan, Quan-Long Yang, Xiao-Ying Wang, Dong-Lin Zhao, Man-Hua Li, Xue-Liang Wu, Jian-Chun Fan, Shou-Bin Ning\",\"doi\":\"10.3881/j.issn.1000-503X.15988\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Objective To investigate the effects of pterostilbene on human colon cancer LoVo cells and study the regulatory mechanism of nuclear factor E2-related factor 2 (Nrf2) in the process of pterostilbene acting on LoVo cells. Methods LoVo cells were treated with different concentrations (5,10,20,40,60,80,100 μmol/L) of pterostilbene.Cell viability,migration,invasion,and apoptosis were examined by CCK-8,scratch,Transwell,and TUNEL assays,respectively.The mitochondrial membrane potential was measured by the mitochondrial membrane potential assay kit with JC-1.The reactive oxygen species level was measured by 2',7'-dichlorofluorescein diacetate.The protein levels of Nrf2,phosphorylated Nrf2,heme oxygenase 1,and apoptotic proteins (Bcl2 and Bax) were determined by Western blotting.In addition,cell viability,Nrf2 expression,and apoptosis rate were determined after co-application of the Nrf2-specific agonist sulforaphane. Results Compared with the control group,40,60,80,100 μmol/L pterostilbene reduced the viability of LoVo cells (<i>P</i>=0.014,<i>P</i><0.001,<i>P</i><0.001,<i>P</i><0.001).Pterostilbene at 5,10,20 μmol/L did not show effects on cell viability but inhibited cell migration (<i>P</i>=0.008,<i>P</i><0.001,<i>P</i><0.001) and invasion (all <i>P</i><0.001).Pterostilbene at 40,60,80 μmol/L increased apoptosis (<i>P</i>=0.014,<i>P</i><0.001,<i>P</i><0.001),promoted mitochondrial membrane potential depolarization (<i>P</i>=0.026,<i>P</i><0.001,<i>P</i><0.001) and reactive oxygen species accumulation (all <i>P</i><0.001),and down-regulated the expression of phosphorylated Nrf2 (<i>P</i>=0.030,<i>P</i><0.001,<i>P</i><0.001),heme oxygenase 1 (<i>P</i>=0.015,<i>P</i><0.001,<i>P</i><0.001),and Bcl2 (<i>P</i>=0.039,<i>P</i><0.001,<i>P</i><0.001) in LoVo cells.Pterostilbene at 60,80 μmol/L down-regulated Nrf2 expression (<i>P</i>=0.001,<i>P</i><0.001) and up-regulated Bax expression (both <i>P</i><0.001).The application of sulforaphane reversed the effects of pterostilbene on cell viability (<i>P</i><0.001),apoptosis (<i>P</i><0.001),and Nrf2 expression (<i>P</i>=0.022). Conclusion Pterostilbene is a compound that can effectively inhibit colon cancer cells by inhibiting the Nrf2 pathway.</p>\",\"PeriodicalId\":6919,\"journal\":{\"name\":\"中国医学科学院学报\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国医学科学院学报\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3881/j.issn.1000-503X.15988\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国医学科学院学报","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3881/j.issn.1000-503X.15988","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Effect of Pterostilbene Regulating Nuclear Factor E2-Related Factor 2 on Apoptosis of Colon Cancer Cells in Vitro].
Objective To investigate the effects of pterostilbene on human colon cancer LoVo cells and study the regulatory mechanism of nuclear factor E2-related factor 2 (Nrf2) in the process of pterostilbene acting on LoVo cells. Methods LoVo cells were treated with different concentrations (5,10,20,40,60,80,100 μmol/L) of pterostilbene.Cell viability,migration,invasion,and apoptosis were examined by CCK-8,scratch,Transwell,and TUNEL assays,respectively.The mitochondrial membrane potential was measured by the mitochondrial membrane potential assay kit with JC-1.The reactive oxygen species level was measured by 2',7'-dichlorofluorescein diacetate.The protein levels of Nrf2,phosphorylated Nrf2,heme oxygenase 1,and apoptotic proteins (Bcl2 and Bax) were determined by Western blotting.In addition,cell viability,Nrf2 expression,and apoptosis rate were determined after co-application of the Nrf2-specific agonist sulforaphane. Results Compared with the control group,40,60,80,100 μmol/L pterostilbene reduced the viability of LoVo cells (P=0.014,P<0.001,P<0.001,P<0.001).Pterostilbene at 5,10,20 μmol/L did not show effects on cell viability but inhibited cell migration (P=0.008,P<0.001,P<0.001) and invasion (all P<0.001).Pterostilbene at 40,60,80 μmol/L increased apoptosis (P=0.014,P<0.001,P<0.001),promoted mitochondrial membrane potential depolarization (P=0.026,P<0.001,P<0.001) and reactive oxygen species accumulation (all P<0.001),and down-regulated the expression of phosphorylated Nrf2 (P=0.030,P<0.001,P<0.001),heme oxygenase 1 (P=0.015,P<0.001,P<0.001),and Bcl2 (P=0.039,P<0.001,P<0.001) in LoVo cells.Pterostilbene at 60,80 μmol/L down-regulated Nrf2 expression (P=0.001,P<0.001) and up-regulated Bax expression (both P<0.001).The application of sulforaphane reversed the effects of pterostilbene on cell viability (P<0.001),apoptosis (P<0.001),and Nrf2 expression (P=0.022). Conclusion Pterostilbene is a compound that can effectively inhibit colon cancer cells by inhibiting the Nrf2 pathway.
期刊介绍：
Acta Academiae Medicinae Sinicae was founded in February 1979. It is a comprehensive medical academic journal published in China and abroad, supervised by the Ministry of Health of the People's Republic of China and sponsored by the Chinese Academy of Medical Sciences and Peking Union Medical College.
The journal mainly reports the latest research results, work progress and dynamics in the fields of basic medicine, clinical medicine, pharmacy, preventive medicine, biomedicine, medical teaching and research, aiming to promote the exchange of medical information and improve the academic level of medicine. At present, the journal has been included in 10 famous foreign retrieval systems and their databases [Medline (PubMed online version), Elsevier, EMBASE, CA, WPRIM, ExtraMED, IC, JST, UPD and EBSCO-ASP]; and has been included in important domestic retrieval systems and databases [China Science Citation Database (Documentation and Information Center of the Chinese Academy of Sciences), China Core Journals Overview (Peking University Library), China Science and Technology Paper Statistical Source Database (China Science and Technology Core Journals) (China Institute of Scientific and Technological Information), China Science and Technology Journal Paper and Citation Database (China Institute of Scientific and Technological Information)].
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