Shermila Kulanayake, Barinder Singh, Faryal Dar, Suresh K Tikoo
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引用次数: 0
摘要
牛腺病毒(BAdV)-3 基因组编码一种 26 kDa 的核心蛋白,称为蛋白 VII,该蛋白定位于细胞核/核仁。VII缺失的BAdV-3病毒的复制需要与蛋白VII互补的细胞系,这表明蛋白VII是产生感染性后代病毒所必需的。对 BAV.VIId+ 病毒(仅在表型上对蛋白 VII 呈阳性)的分析发现,病毒蛋白的表达和在病毒中的结合没有明显差异。此外,蛋白 VII 似乎对成熟 BAV.VIId+ 的形成并不重要。然而,蛋白 VII 似乎是有效组装成熟 BAV.VIId- 病毒所必需的。对非互补细胞中的 BAV.VIId- 病毒(蛋白 VII 的基因型和表型均为阴性)进行的分析发现,病毒从内体释放的效率很低,这影响了病毒蛋白质的表达或 DNA 的复制。此外,蛋白 VII 的缺失改变了 BAV.VIId- 蛋白 VI 的蛋白水解。我们的研究结果表明,BAdV-3蛋白VII似乎是高效产生成熟病毒所必需的。此外,缺乏蛋白 VII 会改变 BAdV-3 从内体/囊泡中的释放,从而产生非感染性 BAdV-3。
Role of Protein VII in the Production of Infectious Bovine Adenovirus-3 Virion.
Bovine adenovirus (BAdV)-3 genome encodes a 26 kDa core protein designated as protein VII, which localizes to the nucleus/nucleolus. The requirement of a protein VII-complementing cell line for the replication of VII-deleted BAdV-3 suggests that protein VII is required for the production of infectious progeny virions. An analysis of the BAV.VIId+ virus (only phenotypically positive for protein VII) detected no noticeable differences in the expression and incorporation of viral proteins in the virions. Moreover, protein VII does not appear to be essential for the formation of mature BAV.VIId+. However, protein VII appeared to be required for the efficient assembly of mature BAV.VIId- virions. An analysis of the BAV.VIId- virus (genotypically and phenotypically negative for protein VII) in non-complementing cells detected the inefficient release of virions from endosomes, which affected the expression of viral proteins or DNA replication. Moreover, the absence of protein VII altered the proteolytic cleavage of protein VI of BAV.VIId-. Our results suggest that BAdV-3 protein VII appears to be required for efficient production of mature virions. Moreover, the absence of protein VII produces non-infectious BAdV-3 by altering the release of BAdV-3 from endosomes/vesicles.
期刊介绍:
Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.