带有典型里德-斯滕伯格细胞的猫霍奇金样淋巴瘤的组织学、表型和分子特征。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Veterinary and comparative oncology Pub Date : 2024-12-01 Epub Date: 2024-08-30 DOI:10.1111/vco.13007
Laura Marconato, Ilaria Maga, Selina Iussich, Silvia Benali, Luca Aresu
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引用次数: 0

摘要

霍奇金样淋巴瘤(HLL)是猫的一种罕见肿瘤,与人类的这种疾病具有相同的特征。HLL 的特征是存在表达 CD30 和 CD20 的 Reed-Sternberg (RS) 细胞。本研究旨在阐明猫 HLL 的临床病理特征、免疫表型和克隆模式。MyLav实验室对6年间结节淋巴瘤的临床病理学和分子数据进行了全面的回顾性研究。共发现 24 例病例。所有病猫都出现了颌下或咽后淋巴结病。组织病理学检查显示,中型到大型淋巴细胞呈多灶性到弥漫性增生,有丝分裂活性低,间有RS细胞,并伴有由T细胞、浆细胞和中性粒细胞组成的异质性炎症浸润。此外,广泛的坏死也是一致的发现。免疫组化显示,肿瘤细胞有不同程度的膜CD20和核PAX5表达,而RS细胞仅有轻度至中度CD20阳性,PAX5阴性。在 21/24 例病例(87.5%)中,RS 细胞呈弥漫性 CD30 阳性。PARR分析表明,60%的病例存在克隆性B细胞扩增,其余40%的病例表现出多克隆性。在随访的 10 只猫中,预后普遍良好,只有两只猫因疾病进展而死亡。总之,猫 HLL 的诊断具有挑战性。RS细胞表达CD30和CD20应被视为该病的特征,但只有在排除了无性B细胞淋巴瘤和肉芽肿性淋巴结病等鉴别诊断后才能确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histologic, Phenotypic, and Molecular Characterization of Feline Hodgkin-Like Lymphoma With Classical Reed-Sternberg Cells.

Hodgkin-like lymphoma (HLL) is a rare neoplasm in cats that shares characteristics with the human disease. The hallmark of HLL is the presence of Reed-Sternberg (RS) cells expressing CD30 and CD20. This study aimed to elucidate the clinicopathologic features, immunophenotype and clonality patterns of feline HLL. A comprehensive retrospective review of clinicopathologic and molecular data of nodal lymphomas over a 6-year period was conducted in MyLav laboratory. Twenty-four cases were identified. All cats presented with submandibular or retropharyngeal lymphadenopathy. Histopathologic examination revealed a multifocal to diffuse proliferation of medium-to-large lymphoid cells with low mitotic activity, interspersed RS cells, and a heterogeneous inflammatory infiltrate comprising T-cells, plasma cells and neutrophils. In addition, extensive necrosis was a consistent finding. Immunohistochemistry showed a variable membranous CD20 and nuclear PAX5 expression in neoplastic cells, while RS cells displayed only mild to moderate CD20 positivity and were negative to PAX5. In 21/24 cases (87.5%), RS cells were diffusely CD30-positive. PARR analysis demonstrated clonal B-cell expansion in 60% of cases, with the remaining 40% exhibiting polyclonality. For the 10 cats with available follow-up, the prognosis was generally favourable, with only two cats succumbing to progressive disease. In conclusion, diagnosing feline HLL is challenging. The expression of CD30 and CD20 by RS cells should be considered a hallmark of the disease, but only after excluding differential diagnoses such as anaplastic B-cell lymphoma and granulomatous lymphadenopathy.

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来源期刊
Veterinary and comparative oncology
Veterinary and comparative oncology 农林科学-兽医学
CiteScore
4.80
自引率
9.50%
发文量
75
审稿时长
>24 weeks
期刊介绍: Veterinary and Comparative Oncology (VCO) is an international, peer-reviewed journal integrating clinical and scientific information from a variety of related disciplines and from worldwide sources for all veterinary oncologists and cancer researchers concerned with aetiology, diagnosis and clinical course of cancer in domestic animals and its prevention. With the ultimate aim of diminishing suffering from cancer, the journal supports the transfer of knowledge in all aspects of veterinary oncology, from the application of new laboratory technology to cancer prevention, early detection, diagnosis and therapy. In addition to original articles, the journal publishes solicited editorials, review articles, commentary, correspondence and abstracts from the published literature. Accordingly, studies describing laboratory work performed exclusively in purpose-bred domestic animals (e.g. dogs, cats, horses) will not be considered.
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