脐带间充质干细胞衍生线粒体移植对大鼠模型缺血再灌注损伤的影响

IF 2 4区医学 Q3 DERMATOLOGY

Skin Research and Technology Pub Date : 2024-09-01 DOI:10.1111/srt.70022

Chan Yeong Lee, Galina Khan, Dong Yun Hyun, Sang Hun Kim, Eun Soo Park

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引用次数: 0

摘要

背景：尽管重建手术取得了进展，但缺血再灌注（I/R）损伤仍是重建手术中的一大挑战，其中线粒体功能障碍起着关键作用。线粒体移植已成为解决这一问题的一种有前途的治疗策略。本研究旨在评估脐带间充质干细胞线粒体移植对大鼠皮瓣I/R模型的影响：20只雄性大鼠接受了皮瓣I/R损伤，通过静脉或皮下途径给予或不给予线粒体移植。分析包括组织病理学、炎症、细胞凋亡、氧化应激和缺氧指标：结果：结果显示，与对照组相比，移植组的炎症、细胞凋亡、氧化应激和缺氧均有所减少：结论：研究结果表明，脐带间充质干细胞衍生线粒体移植有望提高皮瓣存活率，减轻I/R损伤，为改善重建手术效果提供了宝贵的见解。不过，还需要在更大的动物模型中进行进一步探索，并改进给药方法和剂量，以充分阐明其临床可转化性。

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Effect of umbilical cord mesenchymal stem cell-derived mitochondrial transplantation on ischemia-reperfusion injury in a rat model.

Background: Despite advancements in reconstructive procedures, ischemia-reperfusion (I/R) injury remains a significant challenge in reconstructive surgery, with mitochondrial dysfunction playing a pivotal role. Mitochondrial transplantation has emerged as a promising therapeutic strategy to address this issue. This study aims to evaluate the impact of umbilical cord mesenchymal stem cell-derived mitochondrial transplantation on skin flap I/R models in rats.

Material and methods: Twenty male rats underwent I/R injury on skin flaps, with or without mitochondrial transplantation administered via intravenous or subcutaneous routes. Analysis encompassed histopathology, inflammatory, apoptotic, oxidative stress, and hypoxia markers.

Results: Results revealed a reduction in inflammation, apoptosis, oxidative stress, and hypoxia in the transplantation group compared to controls.

Conclusion: The findings suggest that umbilical cord mesenchymal stem cell-derived mitochondrial transplantation shows promise in enhancing flap viability and attenuating I/R injury, offering valuable insights for improved outcomes in reconstructive surgery. However, further exploration in larger animal models and refinement of delivery methods and dosage are warranted to fully elucidate its clinical translatability.

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来源期刊

Skin Research and Technology 医学-皮肤病学

CiteScore

3.30

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.