激酶 CPK10 以钙依赖方式调控弱光诱导的番茄落花(IDL6 的下游)。

IF 6.5 1区 生物学 Q1 PLANT SCIENCES
Xin Fu, Ruizhen Li, Xianfeng Liu, Lina Cheng, Siqi Ge, Sai Wang, Yue Cai, Tong Zhang, Chun-Lin Shi, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Tianlai Li, Mingfang Qi, Tao Xu
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引用次数: 0

摘要

落花是许多作物减产的主要原因。此前,我们发现番茄(Solanum lycopersicum)的类退花因子(SlIDL6)会导致弱光诱导的落花。然而,SlIDL6 作为信号调节弱光诱导的脱落的分子机制仍不清楚。本研究发现,SlIDL6 能提高脱落区(AZ)的细胞膜 Ca2+ 浓度([Ca2+]cyt),而这是 SlIDL6 在弱光下诱导落花所必需的。我们进一步发现,一个钙依赖性蛋白激酶基因(SlCPK10)在脱落带中高表达,并在 SlIDL6 诱导的[Ca2+]cyt 作用下上调。在番茄中过度表达和敲除 SlCPK10 分别会导致加速和延迟脱落。遗传学证据进一步表明,敲除 SlCPK10 会显著削弱 SlIDL6 在加速脱落方面的功能。此外,SlCPK10中依赖于SlIDL6的Ser-371磷酸化是其调节落花功能的必要且充分条件,这可能是通过稳定SlCPK10蛋白实现的。综上所述,我们的研究结果表明,SlCPK10作为IDL6信号通路的下游成分,调节着番茄在弱光胁迫下的落花。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CPK10 regulates low light-induced tomato flower drop downstream of IDL6 in a calcium-dependent manner.

Flower drop is a major cause for yield loss in many crops. Previously, we found that the tomato (Solanum lycopersicum) INFLORESCENCE DEFICIENT IN ABSCISSION-Like (SlIDL6) gene contributes to flower drop induced by low light. However, the molecular mechanisms by which SlIDL6 acts as a signal to regulate low light-induced abscission remain unclear. In this study, SlIDL6 was found to elevate cytosolic Ca2+ concentrations ([Ca2+]cyt) in the abscission zone (AZ), which was required for SlIDL6-induced flower drop under low light. We further identified that 1 calcium-dependent protein kinase gene, SlCPK10, was highly expressed in the AZ and upregulated by SlIDL6-triggered [Ca2+]cyt. Overexpression and knockout of SlCPK10 in tomato resulted in accelerated and delayed abscission, respectively. Genetic evidence further indicated that knockout of SlCPK10 significantly impaired the function of SlIDL6 in accelerating abscission. Furthermore, Ser-371 phosphorylation in SlCPK10 dependent on SlIDL6 was necessary and sufficient for its function in regulating flower drop, probably by stabilizing the SlCPK10 proteins. Taken together, our findings reveal that SlCPK10, as a downstream component of the IDL6 signaling pathway, regulates flower drop in tomato under low-light stress.

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来源期刊
Plant Physiology
Plant Physiology 生物-植物科学
CiteScore
12.20
自引率
5.40%
发文量
535
审稿时长
2.3 months
期刊介绍: Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.
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