变异单胞菌反义 vicK RNA 过度表达加抗菌剂甲基丙烯酸二甲氨基十六烷基酯可抑制口腔生物膜并保护拔出的人类牙齿的珐琅质硬度。

IF 3.3 3区 医学 Q2 MICROBIOLOGY
Shuang Yu, Mengmeng Xu, Zheng Wang, Yang Deng, Hockin H K Xu, Michael D Weir, Negar Homayounfar, Guadalupe Garcia Fay, Hong Chen, Deqin Yang
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引用次数: 0

摘要

变异链球菌(S. mutans)反义 vicK RNA(ASvicK)是一种非编码 RNA,可调节致龋毒性和代谢活动。甲基丙烯酸二甲基氨基十六烷基酯(DMAHDM)是一种用于牙科材料的甲基丙烯酸季铵酯,具有很强的抗菌活性。本研究考察了变异单胞菌 ASvicK 对 DMAHDM 敏感性的影响,以及它们对抑制变异单胞菌生物膜形成和保护牙釉质硬度的综合影响。本研究测试了母体变异棒状杆菌 UA159 和过表达的变异棒状杆菌 ASvicK(ASvicK)。测量了对浮游细菌(MBC-P)和生物膜(MBC-B)的最低抑菌浓度(MIC)和最低杀菌浓度。由于 ASvicK MBC-B 的抑菌浓度为 175 μg/mL,因此在 175 μg/mL 和 87.5 μg/mL 的抑菌浓度下,研究了活/死染色、代谢活性(MTT)、菌落形成单位(CFU)、生物膜生物量、多糖和乳酸的产生。ASvicK 菌株的 DMAHDM MIC、MBC-P 和 MBC-B 值是 UA159 菌株的一半。此外,将 S. mutans ASvicK 与 DMAHDM 结合使用可使 CFU 显著减少 4 个菌落(p < 0.05),多糖含量和乳酸产量也明显减少。在体外致龋模型中,该组合的牙釉质硬度最高,为健全牙釉质的 67.1%,而不含 DMAHDM 的 UA159 的牙釉质硬度最低,仅为 16.4%(p < 0.05)。因此,变异单胞菌 ASvicK 增强了对 DMAHDM 的敏感性,它们的组合能有效抑制生物膜的形成,并将釉质脱矿化降至最低。变异单胞菌 ASvicK + DMAHDM 组合在防龋牙科应用中显示出巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
S. mutans Antisense vicK RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth.

Streptococcus mutans (S. mutans) antisense vicK RNA (ASvicK) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of S. mutans ASvicK on DMAHDM susceptibility and their combined impact on inhibiting S. mutans biofilm formation and protecting enamel hardness. The parent S. mutans UA159 and ASvicK overexpressing S. mutans (ASvicK) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the ASvicK MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the ASvicK strain were half those of the UA159 strain. In addition, combining S. mutans ASvicK with DMAHDM resulted in a significant 4-log CFU reduction (p < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% (p < 0.05). Thus, S. mutans ASvicK enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The S. mutans ASvicK + DMAHDM combination shows great potential for anti-caries dental applications.

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来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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