Shuang Yu, Mengmeng Xu, Zheng Wang, Yang Deng, Hockin H K Xu, Michael D Weir, Negar Homayounfar, Guadalupe Garcia Fay, Hong Chen, Deqin Yang
{"title":"变异单胞菌反义 vicK RNA 过度表达加抗菌剂甲基丙烯酸二甲氨基十六烷基酯可抑制口腔生物膜并保护拔出的人类牙齿的珐琅质硬度。","authors":"Shuang Yu, Mengmeng Xu, Zheng Wang, Yang Deng, Hockin H K Xu, Michael D Weir, Negar Homayounfar, Guadalupe Garcia Fay, Hong Chen, Deqin Yang","doi":"10.3390/pathogens13080707","DOIUrl":null,"url":null,"abstract":"<p><p><i>Streptococcus mutans</i> (<i>S. mutans</i>) antisense <i>vicK</i> RNA (AS<i>vicK</i>) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of <i>S. mutans</i> AS<i>vicK</i> on DMAHDM susceptibility and their combined impact on inhibiting <i>S. mutans</i> biofilm formation and protecting enamel hardness. The parent <i>S. mutans</i> UA159 and AS<i>vicK</i> overexpressing <i>S. mutans</i> (AS<i>vicK</i>) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the AS<i>vicK</i> MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the AS<i>vicK</i> strain were half those of the UA159 strain. In addition, combining <i>S. mutans</i> AS<i>vicK</i> with DMAHDM resulted in a significant 4-log CFU reduction (<i>p</i> < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% (<i>p</i> < 0.05). Thus, <i>S. mutans</i> AS<i>vicK</i> enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The <i>S. mutans</i> AS<i>vicK</i> + DMAHDM combination shows great potential for anti-caries dental applications.</p>","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11356802/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>S. mutans</i> Antisense <i>vicK</i> RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth.\",\"authors\":\"Shuang Yu, Mengmeng Xu, Zheng Wang, Yang Deng, Hockin H K Xu, Michael D Weir, Negar Homayounfar, Guadalupe Garcia Fay, Hong Chen, Deqin Yang\",\"doi\":\"10.3390/pathogens13080707\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Streptococcus mutans</i> (<i>S. mutans</i>) antisense <i>vicK</i> RNA (AS<i>vicK</i>) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of <i>S. mutans</i> AS<i>vicK</i> on DMAHDM susceptibility and their combined impact on inhibiting <i>S. mutans</i> biofilm formation and protecting enamel hardness. The parent <i>S. mutans</i> UA159 and AS<i>vicK</i> overexpressing <i>S. mutans</i> (AS<i>vicK</i>) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the AS<i>vicK</i> MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the AS<i>vicK</i> strain were half those of the UA159 strain. In addition, combining <i>S. mutans</i> AS<i>vicK</i> with DMAHDM resulted in a significant 4-log CFU reduction (<i>p</i> < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% (<i>p</i> < 0.05). Thus, <i>S. mutans</i> AS<i>vicK</i> enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The <i>S. mutans</i> AS<i>vicK</i> + DMAHDM combination shows great potential for anti-caries dental applications.</p>\",\"PeriodicalId\":19758,\"journal\":{\"name\":\"Pathogens\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11356802/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathogens\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3390/pathogens13080707\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/pathogens13080707","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
S. mutans Antisense vicK RNA Over-Expression Plus Antibacterial Dimethylaminohexadecyl Methacrylate Suppresses Oral Biofilms and Protects Enamel Hardness in Extracted Human Teeth.
Streptococcus mutans (S. mutans) antisense vicK RNA (ASvicK) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of S. mutans ASvicK on DMAHDM susceptibility and their combined impact on inhibiting S. mutans biofilm formation and protecting enamel hardness. The parent S. mutans UA159 and ASvicK overexpressing S. mutans (ASvicK) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the ASvicK MBC-B was 175 μg/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 μg/mL and 87.5 μg/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the ASvicK strain were half those of the UA159 strain. In addition, combining S. mutans ASvicK with DMAHDM resulted in a significant 4-log CFU reduction (p < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% (p < 0.05). Thus, S. mutans ASvicK enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The S. mutans ASvicK + DMAHDM combination shows great potential for anti-caries dental applications.
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Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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