功能化单分散微泡生产:快速、可控、自动去除多余涂层材料的微流体方法。

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
M R P van den Broek, M Versluis, A van den Berg, T Segers
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引用次数: 0

摘要

功能化单分散微气泡有望提高分子超声成像的灵敏度和有效性,并利用气泡和超声进行靶向给药。单分散气泡可在微流体流动聚焦装置中产生。然而,气泡的功能化和连续使用需要去除气泡悬浮液中多余的脂质,以尽量减少昂贵配体的使用,避免竞争性结合和受体分子阻塞。迄今为止,去除多余脂质的方法是离心分离,这种方法既耗费人力,又难以实现自动化。更重要的是,正如我们所展示的,离心过程中增加的静水压力会降低气泡的单分散性。在此,我们介绍一种新型的自动化微流控 "清洗 "方法。首先,将气泡注入高度为 1 毫米的微流控室内,让气泡漂浮在顶壁上。其次,用泵将无脂介质泵入微流室,以去除多余的脂质,同时让气泡停留在顶壁。第三,将洗净的气泡重新悬浮并从装置中取出,放入收集瓶中。我们证明,本方法可以:(i) 将过量脂质浓度降低 4 个数量级;(ii) 完全自动化;(iii) 在几分钟内完成,同时气泡的大小分布、功能和声学响应不受影响。因此,所介绍的方法是实现全自动生产功能化单分散微气泡的一个途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Functionalized monodisperse microbubble production: microfluidic method for fast, controlled, and automated removal of excess coating material.

Functionalized monodisperse microbubble production: microfluidic method for fast, controlled, and automated removal of excess coating material.

Functionalized monodisperse microbubbles have the potential to boost the sensitivity and efficacy of molecular ultrasound imaging and targeted drug delivery using bubbles and ultrasound. Monodisperse bubbles can be produced in a microfluidic flow focusing device. However, their functionalization and sequential use require removal of the excess lipids from the bubble suspension to minimize the use of expensive ligands and to avoid competitive binding and blocking of the receptor molecules. To date, excess lipid removal is performed by centrifugation, which is labor intensive and challenging to automate. More importantly, as we show, the increased hydrostatic pressure during centrifugation can reduce bubble monodispersity. Here, we introduce a novel automated microfluidic 'washing' method. First, bubbles are injected in a microfluidic chamber 1 mm in height where they are left to float against the top wall. Second, lipid-free medium is pumped through the chamber to remove excess lipids while the bubbles remain located at the top wall. Third, the washed bubbles are resuspended and removed from the device into a collection vial. We demonstrate that the present method can (i) reduce the excess lipid concentration by 4 orders of magnitude, (ii) be fully automated, and (iii) be performed in minutes while the size distribution, functionality, and acoustic response of the bubbles remain unaffected. Thus, the presented method is a gateway to the fully automated production of functionalized monodisperse microbubbles.

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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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