高血糖处理的 ARPE-19 细胞中炎症体的微 RNA 调节。

IF 1.8 4区 医学 Q3 OPHTHALMOLOGY
Journal of Ophthalmology Pub Date : 2024-08-24 eCollection Date: 2024-01-01 DOI:10.1155/2024/3654690
Ji Hong Kim, Hyoseon Yu, Ji Hye Kang, Eun Hee Hong, Min Ho Kang, Mincheol Seong, Heeyoon Cho, Yong Un Shin
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引用次数: 0

摘要

目的:本研究旨在评估糖尿病诱导的视网膜细胞中微小RNA(miRNA)和炎性体的表达,并确定它们在糖尿病视网膜病变(DR)发病机制中的作用:方法:为了建立糖尿病诱导细胞模型,ARPE-19细胞经高糖处理。方法:为了建立糖尿病诱导的细胞模型,用高血糖处理 ARPE-19 细胞,用实时定量聚合酶链反应法测定高糖处理的 ARPE-19 细胞中五种 miRNA(miR-185、miR-17、miR-20a、miR-15a 和 miR-15b)的表达水平。选择 miR-17 作为目标 miRNA,在高糖处理的 ARPE-19 细胞中转染 miR-17 模拟物后测量炎性体的表达:结果:在高糖处理的 ARPE-19 细胞中,miRNA 的表达大幅下调,而炎症小体成分的表达则显著增加。在高糖处理的 ARPE-19 细胞中转染 miR-17 模拟物后,炎症小体成分的水平明显下降:本研究利用高糖处理的 ARPE-19 细胞研究了糖尿病诱导细胞中 miRNA 与炎性体之间的关系。这些研究结果表明,miR-17 可抑制炎性体,从而减少随后的炎症反应,并表明 miRNA 和炎性体可作为 DR 的新治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MicroRNA Regulation for Inflammasomes in High Glucose-Treated ARPE-19 Cells.

Purpose: This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes-induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR).

Methods: To establish diabetes-induced cell models, ARPE-19 cells were treated with high glucose. The expression levels of five miRNAs (miR-185, miR-17, miR-20a, miR-15a, and miR-15b) were measured in high glucose-treated ARPE-19 cells using real-time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR-17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR-17 mimic into high glucose-treated ARPE-19 cells.

Results: In high glucose-treated ARPE-19 cells, miRNA expression was substantially downregulated, whereas that of inflammasome components was significantly increased. Following the transfection of the miR-17 mimic into high glucose-treated ARPE-19 cells, the levels of inflammasome components were significantly decreased.

Conclusions: This study investigated the relationship between miRNAs and inflammasomes in diabetes-induced cells using high glucose-treated ARPE-19 cells. These findings suggested that miR-17 suppresses inflammasomes, thereby reducing the subsequent inflammatory response and indicating that miRNAs and inflammasomes could serve as new therapeutic targets for DR.

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来源期刊
Journal of Ophthalmology
Journal of Ophthalmology MEDICINE, RESEARCH & EXPERIMENTAL-OPHTHALMOLOGY
CiteScore
4.30
自引率
5.30%
发文量
194
审稿时长
6-12 weeks
期刊介绍: Journal of Ophthalmology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies related to the anatomy, physiology and diseases of the eye. Submissions should focus on new diagnostic and surgical techniques, instrument and therapy updates, as well as clinical trials and research findings.
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