Sparassis latifolia 通过抑制 3T3-L1 细胞脂肪生成和激活脂肪分解抑制脂肪积累的活性

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-10-28 Epub Date: 2024-07-30 DOI:10.4014/jmb.2404.04037
Jeong Won Choi, Hyeok Jin Choi, Rhim Ryoo, Youngki Park, Kyoung Tae Lee, Jin Boo Jeong
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引用次数: 0

摘要

据报道,Sparassis latifolia(SL)在高脂饮食动物模型中表现出抗肥胖作用,但对其作用机制的研究仍然有限。因此,本研究旨在利用 3T3-L1 细胞在体外环境中阐明白花蛇舌草 30% 乙醇提取物(SL30E)抗肥胖活性的机制。SL30E 能有效缓解脂滴和三酰甘油的积累。SL30E 下调了 PPARγ 和 CEBPα 蛋白水平。使用β-catenins特异性siRNA敲除β-catenins会阻碍SL30E对PPARγ和C/EBPα蛋白水平的降低。此外,还观察到SL30E增加了ATGL和p-HSL的蛋白水平,同时降低了perilipin-1的蛋白水平。SL30E 下调了 p62/SQSTM1 蛋白水平,上调了 LC3-II 蛋白水平。此外,SL30E 还能提高 p-AMPK 和 PGC-1α 的蛋白水平。结果表明,SL30E 通过抑制 3T3-L1 细胞的脂肪生成、诱导脂肪分解、脂肪吞噬和产热来抑制脂质积累。这些观察结果为了解 SL 的抗肥胖作用机制提供了潜在的见解,为现有的知识体系提供了宝贵的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory Activity of Sparassis latifolia on the Lipid Accumulation through Suppressing Adipogenesis and Activating Lipolysis in 3T3-L1 Cells.

Sparassis latifolia (SL) has been reported to exhibit anti-obesity effects in high-fat diet animal models, yet research into its mechanisms of action remains limited. Therefore, this study aimed to elucidate the mechanisms behind the anti-obesity activity of SL's 30% ethanol extract (SL30E) using 3T3-L1 cells in an in vitro setting. SL30E effectively mitigated the accumulation of lipid droplets and triacylglycerol. SL30E downregulated PPARγ and CEBPα protein levels. The diminishment of PPARγ and C/EBPα, facilitated by SL30E, was impeded by the knockdown of β-catenin using β-catenin-specific siRNA. Furthermore, SL30E was observed to increase the protein levels of ATGL and p-HSL, while it concurrently decreased the protein levels of perilipin-1. SL30E downregulated p62/SQSTM1 protein level and upregulated LC3-II protein level. Moreover, SL30E was demonstrated to elevate the protein levels of p-AMPK and PGC-1α. The results indicate that SL30E inhibits lipid accumulation by suppressing adipogenesis and inducing lipolysis, lipophagy, and thermogenesis in 3T3-L1 cells. These observations provide potential insights into the mechanisms underlying the anti-obesity effects of SL, contributing valuable information to the existing body of knowledge.

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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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