Alicia G Gubieda, Lucía Gandarias, Mihály Pósfai, Ajith Pattammattel, M Luisa Fdez-Gubieda, Ana Abad-Díaz-de-Cerio, Ana García-Prieto
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引用次数: 0
摘要
从细胞成像到癌症治疗,磁性纳米粒子为生物医学提供了许多令人兴奋的可能性。磁小体是目前研究的纳米粒子之一,它是由趋磁细菌合成的化学纯度很高的磁铁矿纳米粒子。尽管磁小体具有治疗潜力,但人们对其在人体细胞内的降解情况知之甚少,对其在肿瘤内的降解情况更是知之甚少。为了探索磁小体治疗癌症的潜力,我们在三维人类肺癌模型中,以纳米级分辨率在亚细胞水平上探索了磁小体的降解过程。我们使用了最先进的硬 X 射线探针(纳米 XANES 和纳米 XRF),可识别细胞各区域中不同的铁相。我们的研究结果揭示了磁小体内磁铁矿氧化成镁铁矿的过程,以及铁蛋白对磁铁矿和铁血黄铜的生物合成过程。
Temporal and spatial resolution of magnetosome degradation at the subcellular level in a 3D lung carcinoma model.
Magnetic nanoparticles offer many exciting possibilities in biomedicine, from cell imaging to cancer treatment. One of the currently researched nanoparticles are magnetosomes, magnetite nanoparticles of high chemical purity synthesized by magnetotactic bacteria. Despite their therapeutic potential, very little is known about their degradation in human cells, and even less so of their degradation within tumours. In an effort to explore the potential of magnetosomes for cancer treatment, we have explored their degradation process in a 3D human lung carcinoma model at the subcellular level and with nanometre scale resolution. We have used state of the art hard X-ray probes (nano-XANES and nano-XRF), which allow for identification of distinct iron phases in each region of the cell. Our results reveal the progression of magnetite oxidation to maghemite within magnetosomes, and the biosynthesis of magnetite and ferrihydrite by ferritin.
期刊介绍:
Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.