{"title":"Z 光谱核磁共振成像通过脂肪-水-比例和酰胺-质子转移对比来量化脂肪组织的质量和代谢活动。","authors":"Zimeng Cai, Qiaoling Zhong, Daming Zhang, Yanqiu Feng, Qian Wang, Yuanbo Yang, Yongzhou Xu, Changhong Liang, Zaiyi Liu, Kejia Cai","doi":"10.1002/jmri.29598","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Brown adipose tissue (BAT) is metabolically activatable and plays an important role in obesity and metabolic diseases. With reduced fat-water-fraction (FWF) compared with white adipose tissue (WAT), BAT mass and its functional activation may be quantified with Z-spectra MRI, with built-in FWF and the metabolic amide proton transfer (APT) contrasts.</p><p><strong>Purpose: </strong>To investigate if Z-spectral MRI can quantify the mass and metabolic activity of adipose tissues.</p><p><strong>Study type: </strong>Prospective.</p><p><strong>Subjects: </strong>Seven groups of 8-week-old male rats, including two groups (n = 7 per group) for in vivo MRI study and five groups (n = 5 per group) for ex vivo validation; 12 young and healthy volunteers with 6 male and 6 female.</p><p><strong>Field strength/sequence: </strong>The 7 T small animal and 3 T clinical systems, T<sub>2</sub>-weighted imaging, Rapid Acquisition with Relaxation Enhancement (RARE) readout based chemical exchange saturation transfer (CEST) Z-spectral MRI sequence.</p><p><strong>Assessment: </strong>Quantified FWF and APT from Z-spectra in rats before and after norepinephrine (NE) stimulation and in healthy human subjects; ex vivo measurements of total proteins in BAT from rats.</p><p><strong>Statistical tests: </strong>Two-tailed unpaired Student's t-tests and repeated measures ANOVA. P-value <0.05 was considered significant.</p><p><strong>Results: </strong>Decreased FWF (from 39.6% ± 7.2% before NE injection to 16.4% ± 7.2% 120 minutes after NE injection, P < 0.0001) and elevated APT (from 1.1% ± 0.5% before NE injection to 2.9% ± 0.5% 120 minutes after NE injection, P < 0.0001) signals in BAT were observed with in vivo Z-spectral MRI in rats injected with NE at 7 T MRI. At clinical 3 T, Z-spectral MRI was used to quantify the FWF (58.5% ± 7.2% in BAT and 73.7% ± 6.5% in WAT with P < 0.0001) and APT (2.6% ± 0.8% in BAT and 0.9% ± 0.3% in WAT with P < 0.0001) signals in healthy volunteers. APT signals of BAT were negatively correlated with the BMI in humans (r = 0.71).</p><p><strong>Data conclusion: </strong>Endogenous Z-spectral MRI was demonstrated to simultaneously quantify BAT mass and function based on its FWF and APT contrasts.</p><p><strong>Level of evidence: 2: </strong></p><p><strong>Technical efficacy stage: </strong>1.</p>","PeriodicalId":16140,"journal":{"name":"Journal of Magnetic Resonance Imaging","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Z-Spectral MRI Quantifies the Mass and Metabolic Activity of Adipose Tissues With Fat-Water-Fraction and Amide-Proton-Transfer Contrasts.\",\"authors\":\"Zimeng Cai, Qiaoling Zhong, Daming Zhang, Yanqiu Feng, Qian Wang, Yuanbo Yang, Yongzhou Xu, Changhong Liang, Zaiyi Liu, Kejia Cai\",\"doi\":\"10.1002/jmri.29598\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Brown adipose tissue (BAT) is metabolically activatable and plays an important role in obesity and metabolic diseases. With reduced fat-water-fraction (FWF) compared with white adipose tissue (WAT), BAT mass and its functional activation may be quantified with Z-spectra MRI, with built-in FWF and the metabolic amide proton transfer (APT) contrasts.</p><p><strong>Purpose: </strong>To investigate if Z-spectral MRI can quantify the mass and metabolic activity of adipose tissues.</p><p><strong>Study type: </strong>Prospective.</p><p><strong>Subjects: </strong>Seven groups of 8-week-old male rats, including two groups (n = 7 per group) for in vivo MRI study and five groups (n = 5 per group) for ex vivo validation; 12 young and healthy volunteers with 6 male and 6 female.</p><p><strong>Field strength/sequence: </strong>The 7 T small animal and 3 T clinical systems, T<sub>2</sub>-weighted imaging, Rapid Acquisition with Relaxation Enhancement (RARE) readout based chemical exchange saturation transfer (CEST) Z-spectral MRI sequence.</p><p><strong>Assessment: </strong>Quantified FWF and APT from Z-spectra in rats before and after norepinephrine (NE) stimulation and in healthy human subjects; ex vivo measurements of total proteins in BAT from rats.</p><p><strong>Statistical tests: </strong>Two-tailed unpaired Student's t-tests and repeated measures ANOVA. P-value <0.05 was considered significant.</p><p><strong>Results: </strong>Decreased FWF (from 39.6% ± 7.2% before NE injection to 16.4% ± 7.2% 120 minutes after NE injection, P < 0.0001) and elevated APT (from 1.1% ± 0.5% before NE injection to 2.9% ± 0.5% 120 minutes after NE injection, P < 0.0001) signals in BAT were observed with in vivo Z-spectral MRI in rats injected with NE at 7 T MRI. At clinical 3 T, Z-spectral MRI was used to quantify the FWF (58.5% ± 7.2% in BAT and 73.7% ± 6.5% in WAT with P < 0.0001) and APT (2.6% ± 0.8% in BAT and 0.9% ± 0.3% in WAT with P < 0.0001) signals in healthy volunteers. APT signals of BAT were negatively correlated with the BMI in humans (r = 0.71).</p><p><strong>Data conclusion: </strong>Endogenous Z-spectral MRI was demonstrated to simultaneously quantify BAT mass and function based on its FWF and APT contrasts.</p><p><strong>Level of evidence: 2: </strong></p><p><strong>Technical efficacy stage: </strong>1.</p>\",\"PeriodicalId\":16140,\"journal\":{\"name\":\"Journal of Magnetic Resonance Imaging\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Magnetic Resonance Imaging\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1002/jmri.29598\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Magnetic Resonance Imaging","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1002/jmri.29598","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
引用次数: 0
摘要
背景：棕色脂肪组织（BAT）可活化代谢，在肥胖和代谢性疾病中发挥着重要作用。与白色脂肪组织（WAT）相比，棕色脂肪组织的脂肪-水比例（FWF）降低，因此可通过内置的 FWF 和代谢酰胺质子转移（APT）对比，用 Z 光谱 MRI 对棕色脂肪组织的质量及其功能激活进行量化：研究对象7组8周大的雄性大鼠，其中2组（每组n=7）用于体内磁共振成像研究，5组（每组n=5）用于体外验证；12名年轻健康的志愿者，其中6男6女：场强/序列：7 T 小动物和 3 T 临床系统，T2 加权成像，基于化学交换饱和转移（CEST）Z 光谱 MRI 序列的弛豫增强快速采集（RARE）读出：通过去甲肾上腺素（NE）刺激前后的 Z 光谱对大鼠和健康人的 FWF 和 APT 进行量化；对大鼠 BAT 中的总蛋白质进行体外测量：双尾非配对学生 t 检验和重复测量方差分析。P 值结果：FWF 下降（从 NE 注射前的 39.6% ± 7.2% 降至 NE 注射 120 分钟后的 16.4% ± 7.2%，P 数据结论：证据级别：2：技术功效阶段：1。
Z-Spectral MRI Quantifies the Mass and Metabolic Activity of Adipose Tissues With Fat-Water-Fraction and Amide-Proton-Transfer Contrasts.
Background: Brown adipose tissue (BAT) is metabolically activatable and plays an important role in obesity and metabolic diseases. With reduced fat-water-fraction (FWF) compared with white adipose tissue (WAT), BAT mass and its functional activation may be quantified with Z-spectra MRI, with built-in FWF and the metabolic amide proton transfer (APT) contrasts.
Purpose: To investigate if Z-spectral MRI can quantify the mass and metabolic activity of adipose tissues.
Study type: Prospective.
Subjects: Seven groups of 8-week-old male rats, including two groups (n = 7 per group) for in vivo MRI study and five groups (n = 5 per group) for ex vivo validation; 12 young and healthy volunteers with 6 male and 6 female.
Field strength/sequence: The 7 T small animal and 3 T clinical systems, T2-weighted imaging, Rapid Acquisition with Relaxation Enhancement (RARE) readout based chemical exchange saturation transfer (CEST) Z-spectral MRI sequence.
Assessment: Quantified FWF and APT from Z-spectra in rats before and after norepinephrine (NE) stimulation and in healthy human subjects; ex vivo measurements of total proteins in BAT from rats.
Statistical tests: Two-tailed unpaired Student's t-tests and repeated measures ANOVA. P-value <0.05 was considered significant.
Results: Decreased FWF (from 39.6% ± 7.2% before NE injection to 16.4% ± 7.2% 120 minutes after NE injection, P < 0.0001) and elevated APT (from 1.1% ± 0.5% before NE injection to 2.9% ± 0.5% 120 minutes after NE injection, P < 0.0001) signals in BAT were observed with in vivo Z-spectral MRI in rats injected with NE at 7 T MRI. At clinical 3 T, Z-spectral MRI was used to quantify the FWF (58.5% ± 7.2% in BAT and 73.7% ± 6.5% in WAT with P < 0.0001) and APT (2.6% ± 0.8% in BAT and 0.9% ± 0.3% in WAT with P < 0.0001) signals in healthy volunteers. APT signals of BAT were negatively correlated with the BMI in humans (r = 0.71).
Data conclusion: Endogenous Z-spectral MRI was demonstrated to simultaneously quantify BAT mass and function based on its FWF and APT contrasts.
期刊介绍：
The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.
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