A V Trofimov, T I Vlasova, V A Trofimov, D I Sidorov, M A Spirina
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引用次数: 0
摘要
拉曼光谱研究了不同严重程度缺血性中风（IS）病例中 DNA 和蛋白质生物大分子的构象变化。研究发现，IS 患者体内血卟啉的构象结构发生了变化，导致（I1355/I1550）/（I1375/I1580）比率（血红蛋白对配体的亲和力）升高，I1375/I1172 增加（吡咯构象发生变化）。基因组 DNA 光谱的变化出现在伯胺（3400 cm-1）、仲胺和参与氢键的羟基（3100 cm-1）、糖磷酸盐的 CH2 基团（2900 cm-1）以及含氮碱基和糖之间的振动键（1400 cm-1）的伸缩振动频率上。在基因组 DNA 和血红蛋白光谱中观察到的明显变化被认为表明了分子在 IS 中的构象重排。严重的 IS 与 DNA 和血红蛋白光谱的最大变化有关。
Conformational Changes in DNA and Protein Biomolecules in Pathogenesis of Ischemic Stroke.
Conformational changes in DNA and protein biomolecules were studied in ischemic stroke (IS) cases varying severity by Raman spectroscopy. The conformational structure of hematoporphyrin was found to change in IS patients, leading to a higher (I1355/I1550)/(I1375/I1580) ratio (hemoglobin affinity of for ligands) and an increase in I1375/I1172 (a change in pyrrole conformation). Changes in genomic DNA spectra were observed at frequencies caused by stretching vibrations of primary amines (3400 cm-1), secondary amines and hydroxyls involved in hydrogen bonding (3100 cm-1), and CH2 groups of sugar phosphates (2900 cm-1) and vibrations of vibrational bonds between nitrogenous bases and sugars (1400 cm-1). The significant changes observed in genomic DNA and hemoglobin spectra were assumed to indicate conformational rearrangements of the molecules in IS. Severe IS was associated with maximum changes in DNA and hemoglobin spectra.
期刊介绍：
Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.
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