Fernando Victor Monteiro Portela, Ana Raquel Colares de Andrade, Lívia Maria Galdino Pereira, Bruno Nascimento da Silva, Paulo Henrique Soares Peixoto, Bruno Rocha Amando, Nicole de Mello Fiallos, Pedro de Freitas Santos Manzi de Souza, Reginaldo Gonçalves de Lima-Neto, Glaucia Morgana de Melo Guedes, Débora Souza Collares Maia Castelo-Branco, Rossana de Aguiar Cordeiro
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引用次数: 0
摘要
白色念珠菌侵袭性念珠菌病被认为是一个全球性的健康问题。在这种情况下，植入装置上形成的生物膜是一种治疗挑战，而这些群落中存在的代谢不活跃的持久细胞（PC）会增加它们对杀真菌药物的耐受性。本研究调查了阿莫西林（AMX）、头孢吡肟（CEF）、庆大霉素（GEN）、阿米卡星（AMK）、万古霉素（VAN）和环丙沙星（CIP）对白僵菌血流分离株生物膜中 PC 的产生的影响。用 100 μg ml-1 两性霉素 B 处理在添加了抗生素的 RPMI-1640 中生长 48 小时的成熟生物膜（n = 6），然后对其进行 PCs 评估。在有抗生素存在的情况下生长的生物膜在接触 AMX 和 CIP 时产生的 PCs 多达 10 倍；接触 CEF 时为 5 倍；接触 GEN 和 VAN 时为 6 倍。结果表明，抗生素可调节白僵菌生物膜中 PC 的产生。这种情况可能会对接受广谱抗生素治疗的免疫力低下患者产生临床影响。
Antibiotics stimulates the development of persistent cells in biofilms of Candida albicans bloodstream isolates.
Candida albicans invasive candidiasis is considered a global health problem. In such cases, biofilm formation on implanted devices represents a therapeutic challenge and the presence of metabolically inactive persistent cells (PCs) in these communities increases their tolerance to fungicidal drugs. This study investigated the influence of amoxicillin, AMX; cefepime, CEF; gentamicin, GEN; amikacin, AMK; vancomycin, VAN; and ciprofloxacin, CIP; on the production of PCs in biofilms of C. albicans bloodstream isolates. 48 h-mature biofilms (n = 6) grown in RPMI-1640 supplemented with antibiotics were treated with 100 μg ml-1 amphotericin B and then evaluated for PCs. Biofilms grown in the presence of antibiotics produced more PCs, up to 10×, when exposed to AMX and CIP; 5 × to CEF; and 6 × to GEN and VAN. The results indicate that antibiotics can modulate PC production in C. albicans biofilms. This scenario may have clinical repercussions in immunocompromised patients under broad-spectrum antibiotic therapy.
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