Maria Soledad Hidrobo , Marcus Höring , Sarah Brunner , Gerhard Liebisch , Sabine Schweizer , Martin Klingenspor , Renate Schreiber , Rudolf Zechner , Ralph Burkhardt , Josef Ecker
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Particularly, the concentrations of PE containing polyunsaturated fatty acids (PUFA) in their acyl chains like PE 18:0_20:4 were increased at cold. Investigation of the plasma's FA profile using gas chromatography coupled to mass spectrometry revealed a negative correlation of PUFA with unsaturated PE in liver and BAT indicating a flux of FA from the circulation into these tissues. Beta-adrenergic stimulation elevated intracellular levels of PE 38:4 and PE 40:6 in beige wildtype adipocytes, but not in adipose triglyceride lipase (ATGL)-deficient cells. These results imply an induction of PE synthesis in liver, BAT and thermogenic adipocytes after activation of the beta-adrenergic signaling cascade.</p></div>","PeriodicalId":8815,"journal":{"name":"Biochimica et biophysica acta. 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引用次数: 0
摘要
通过冷诱导脂肪分解来增加棕色脂肪组织(BAT)的能量消耗被认为是一种潜在的策略,可用于对抗不健康的生活方式和心脏代谢疾病导致的脂质平衡失调。然而,目前还不清楚释放的脂肪酸(FA)是如何代谢的。我们利用基于质谱的定量脂质组学研究了在恒温、23 °C和4 °C条件下饲养1周的小鼠的肝脏和BAT脂质体。在低于恒温的温度下饲养会引发两种组织中磷脂酰乙醇胺(PE)的生成。尤其是在低温条件下,磷脂酰链中含有多不饱和脂肪酸(PUFA)(如 PE 18:0_20:4)的磷脂酰乙醇胺的浓度增加。利用气相色谱-质谱联用技术对血浆中的不饱和脂肪酸概况进行的调查显示,肝脏和 BAT 中的多不饱和脂肪酸与不饱和 PE 呈负相关,这表明不饱和脂肪酸从血液循环进入了这些组织。β-肾上腺素能刺激会提高米色野生型脂肪细胞中 PE 38:4 和 PE 40:6 的细胞内水平,但不会提高脂肪甘油三酯脂酶(ATGL)缺陷细胞中的水平。这些结果表明,β-肾上腺素能信号级联激活后,会诱导肝脏、BAT 和生热脂肪细胞中的 PE 合成。
Cold-induced phosphatidylethanolamine synthesis in liver and brown adipose tissue of mice
Increasing energy expenditure in brown adipose (BAT) tissue by cold-induced lipolysis is discussed as a potential strategy to counteract imbalanced lipid homeostasis caused through unhealthy lifestyle and cardiometabolic disease. Yet, it is largely unclear how liberated fatty acids (FA) are metabolized. We investigated the liver and BAT lipidome of mice housed for 1 week at thermoneutrality, 23 °C and 4 °C using quantitative mass spectrometry-based lipidomics. Housing at temperatures below thermoneutrality triggered the generation of phosphatidylethanolamine (PE) in both tissues. Particularly, the concentrations of PE containing polyunsaturated fatty acids (PUFA) in their acyl chains like PE 18:0_20:4 were increased at cold. Investigation of the plasma's FA profile using gas chromatography coupled to mass spectrometry revealed a negative correlation of PUFA with unsaturated PE in liver and BAT indicating a flux of FA from the circulation into these tissues. Beta-adrenergic stimulation elevated intracellular levels of PE 38:4 and PE 40:6 in beige wildtype adipocytes, but not in adipose triglyceride lipase (ATGL)-deficient cells. These results imply an induction of PE synthesis in liver, BAT and thermogenic adipocytes after activation of the beta-adrenergic signaling cascade.
期刊介绍:
BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.