Mohan R. Badhe, Priyanka Das, Sonalina Sahoo, Anirban Paul, Pramoda Kumar Sahoo, R. Rajendra Kumar Reddy, Amol R. Suryawanshi, Priyanka C. Nandanpawar, Kanta Das Mahapatra, Naresh S. Nagpure, Mukunda Goswami, Jyotirmaya Mohanty
{"title":"罗非鱼对急性热应激的生理反应:肝脏蛋白质组学的启示","authors":"Mohan R. Badhe, Priyanka Das, Sonalina Sahoo, Anirban Paul, Pramoda Kumar Sahoo, R. Rajendra Kumar Reddy, Amol R. Suryawanshi, Priyanka C. Nandanpawar, Kanta Das Mahapatra, Naresh S. Nagpure, Mukunda Goswami, Jyotirmaya Mohanty","doi":"10.1007/s10126-024-10360-6","DOIUrl":null,"url":null,"abstract":"<div><p>Heat stress is a major problem in aquaculture species, causing changes in physiology such as decreased feed intake, growth rate, reproduction, and internal cellular damage, thereby affecting fish’s health. The effects of an acute heat stress simulating a daily rise and fall in temperature on summer days were evaluated in the liver proteome of rohu (<i>Labeo rohita</i>) fingerlings in the present study. The fish maintained at 30 °C were gradually exposed to a higher temperature of 36 °C at an increment rate of 1 °C per 1.5 h, and after 3 h at that temperature, it was gradually reduced to 30 °C. The liver tissue samples were collected at 5 am, 5 pm, and 5 am the next day from the exposed and control fish. Protein samples were prepared from the liver tissues, and the extracted proteins were compared using 2-dimensional (2D) gel electrophoresis (2DGE) and mass spectrometry (MS) using a MALDI-TOF/TOF mass spectrometer. A total of 44 differentially expressed protein spots were visualized in 2D gel analysis from heat stress exposed fish at three time points, out of which 21 proteins including one hypothetical protein could be identified by MS. The abundance of five selected differentially expressed proteins (DEPs) was validated using qPCR. The majority of DEPs were found to be involved primarily in lipid, protein and energy metabolism, immune system regulation, cytoskeletal stability, and ROS management. The findings of this study would help in the development of strategies to mitigate heat stress in <i>L. rohita</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 6","pages":"1129 - 1142"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Physiological Responses to Acute Heat Stress in Rohu, Labeo rohita: Insights from Liver Proteomics\",\"authors\":\"Mohan R. Badhe, Priyanka Das, Sonalina Sahoo, Anirban Paul, Pramoda Kumar Sahoo, R. Rajendra Kumar Reddy, Amol R. Suryawanshi, Priyanka C. Nandanpawar, Kanta Das Mahapatra, Naresh S. Nagpure, Mukunda Goswami, Jyotirmaya Mohanty\",\"doi\":\"10.1007/s10126-024-10360-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Heat stress is a major problem in aquaculture species, causing changes in physiology such as decreased feed intake, growth rate, reproduction, and internal cellular damage, thereby affecting fish’s health. The effects of an acute heat stress simulating a daily rise and fall in temperature on summer days were evaluated in the liver proteome of rohu (<i>Labeo rohita</i>) fingerlings in the present study. The fish maintained at 30 °C were gradually exposed to a higher temperature of 36 °C at an increment rate of 1 °C per 1.5 h, and after 3 h at that temperature, it was gradually reduced to 30 °C. The liver tissue samples were collected at 5 am, 5 pm, and 5 am the next day from the exposed and control fish. Protein samples were prepared from the liver tissues, and the extracted proteins were compared using 2-dimensional (2D) gel electrophoresis (2DGE) and mass spectrometry (MS) using a MALDI-TOF/TOF mass spectrometer. A total of 44 differentially expressed protein spots were visualized in 2D gel analysis from heat stress exposed fish at three time points, out of which 21 proteins including one hypothetical protein could be identified by MS. The abundance of five selected differentially expressed proteins (DEPs) was validated using qPCR. The majority of DEPs were found to be involved primarily in lipid, protein and energy metabolism, immune system regulation, cytoskeletal stability, and ROS management. 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Physiological Responses to Acute Heat Stress in Rohu, Labeo rohita: Insights from Liver Proteomics
Heat stress is a major problem in aquaculture species, causing changes in physiology such as decreased feed intake, growth rate, reproduction, and internal cellular damage, thereby affecting fish’s health. The effects of an acute heat stress simulating a daily rise and fall in temperature on summer days were evaluated in the liver proteome of rohu (Labeo rohita) fingerlings in the present study. The fish maintained at 30 °C were gradually exposed to a higher temperature of 36 °C at an increment rate of 1 °C per 1.5 h, and after 3 h at that temperature, it was gradually reduced to 30 °C. The liver tissue samples were collected at 5 am, 5 pm, and 5 am the next day from the exposed and control fish. Protein samples were prepared from the liver tissues, and the extracted proteins were compared using 2-dimensional (2D) gel electrophoresis (2DGE) and mass spectrometry (MS) using a MALDI-TOF/TOF mass spectrometer. A total of 44 differentially expressed protein spots were visualized in 2D gel analysis from heat stress exposed fish at three time points, out of which 21 proteins including one hypothetical protein could be identified by MS. The abundance of five selected differentially expressed proteins (DEPs) was validated using qPCR. The majority of DEPs were found to be involved primarily in lipid, protein and energy metabolism, immune system regulation, cytoskeletal stability, and ROS management. The findings of this study would help in the development of strategies to mitigate heat stress in L. rohita.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.