{"title":"通过 XRF 分析测量近红外光生物调节疗法对 50B11 感觉神经元离子含量的影响","authors":"Luisa Zupin , Alessandra Gianoncelli , Fulvio Celsi , Valentina Bonanni , George Kourousias , Pietro Parisse , Murielle Salomé , Sergio Crovella , Egidio Barbi , Giuseppe Ricci , Lorella Pascolo","doi":"10.1016/j.jphotobiol.2024.113019","DOIUrl":null,"url":null,"abstract":"<div><p>Photobiomodulation therapy (PBMT) is a form of treatment commonly used for routine clinical applications, such as wound healing of the skin and reduction of inflammation. Additionally, PBMT has been explored for its potential in pain relief.</p><p>In this work, we investigated the effect of PBMT on ion content within the 50B11 sensory neurons cell line in vitro using X-Ray fluorescence (XRF) and atomic force microscope (AFM) analysis.</p><p>Two irradiation protocols were selected utilizing near-infrared laser lights at 800 and 970 nm, with cell fixation immediately following irradiation.</p><p>Results showed a decrease in Calcium content after irradiation with both protocols, and with lidocaine, used as an analgesic control. Furthermore, a reduction in Potassium content was observed, particularly evident when normalized to cellular volume.</p><p>These findings provide valuable insights into the molecular impact of PBMT within 50B11 sensory neurons under normal conditions. Such understanding may contribute to the wider adoption of PBMT as a therapeutic approach.</p></div>","PeriodicalId":16772,"journal":{"name":"Journal of photochemistry and photobiology. B, Biology","volume":"259 ","pages":"Article 113019"},"PeriodicalIF":3.9000,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1011134424001799/pdfft?md5=da1fea496ddc549ae3e55bc26d9bfed5&pid=1-s2.0-S1011134424001799-main.pdf","citationCount":"0","resultStr":"{\"title\":\"The effect of near-infrared Photobiomodulation therapy on the ion content of 50B11 sensory neurons measured through XRF analysis\",\"authors\":\"Luisa Zupin , Alessandra Gianoncelli , Fulvio Celsi , Valentina Bonanni , George Kourousias , Pietro Parisse , Murielle Salomé , Sergio Crovella , Egidio Barbi , Giuseppe Ricci , Lorella Pascolo\",\"doi\":\"10.1016/j.jphotobiol.2024.113019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Photobiomodulation therapy (PBMT) is a form of treatment commonly used for routine clinical applications, such as wound healing of the skin and reduction of inflammation. Additionally, PBMT has been explored for its potential in pain relief.</p><p>In this work, we investigated the effect of PBMT on ion content within the 50B11 sensory neurons cell line in vitro using X-Ray fluorescence (XRF) and atomic force microscope (AFM) analysis.</p><p>Two irradiation protocols were selected utilizing near-infrared laser lights at 800 and 970 nm, with cell fixation immediately following irradiation.</p><p>Results showed a decrease in Calcium content after irradiation with both protocols, and with lidocaine, used as an analgesic control. Furthermore, a reduction in Potassium content was observed, particularly evident when normalized to cellular volume.</p><p>These findings provide valuable insights into the molecular impact of PBMT within 50B11 sensory neurons under normal conditions. Such understanding may contribute to the wider adoption of PBMT as a therapeutic approach.</p></div>\",\"PeriodicalId\":16772,\"journal\":{\"name\":\"Journal of photochemistry and photobiology. B, Biology\",\"volume\":\"259 \",\"pages\":\"Article 113019\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1011134424001799/pdfft?md5=da1fea496ddc549ae3e55bc26d9bfed5&pid=1-s2.0-S1011134424001799-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of photochemistry and photobiology. B, Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1011134424001799\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of photochemistry and photobiology. B, Biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1011134424001799","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
The effect of near-infrared Photobiomodulation therapy on the ion content of 50B11 sensory neurons measured through XRF analysis
Photobiomodulation therapy (PBMT) is a form of treatment commonly used for routine clinical applications, such as wound healing of the skin and reduction of inflammation. Additionally, PBMT has been explored for its potential in pain relief.
In this work, we investigated the effect of PBMT on ion content within the 50B11 sensory neurons cell line in vitro using X-Ray fluorescence (XRF) and atomic force microscope (AFM) analysis.
Two irradiation protocols were selected utilizing near-infrared laser lights at 800 and 970 nm, with cell fixation immediately following irradiation.
Results showed a decrease in Calcium content after irradiation with both protocols, and with lidocaine, used as an analgesic control. Furthermore, a reduction in Potassium content was observed, particularly evident when normalized to cellular volume.
These findings provide valuable insights into the molecular impact of PBMT within 50B11 sensory neurons under normal conditions. Such understanding may contribute to the wider adoption of PBMT as a therapeutic approach.
期刊介绍：
The Journal of Photochemistry and Photobiology B: Biology provides a forum for the publication of papers relating to the various aspects of photobiology, as well as a means for communication in this multidisciplinary field.
The scope includes:
- Bioluminescence
- Chronobiology
- DNA repair
- Environmental photobiology
- Nanotechnology in photobiology
- Photocarcinogenesis
- Photochemistry of biomolecules
- Photodynamic therapy
- Photomedicine
- Photomorphogenesis
- Photomovement
- Photoreception
- Photosensitization
- Photosynthesis
- Phototechnology
- Spectroscopy of biological systems
- UV and visible radiation effects and vision.
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