应激代谢对芽孢杆菌生理生化反应和多环芳烃降解能力的影响

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Bo Zhang , Lei Wang , Zhenjun Diwu , Maiqian Nie , Hongyun Nie
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引用次数: 0

摘要

地衣芽孢杆菌 T5 菌株是从受原油污染的土壤中分离出来的,因为它能有效降解多环芳烃(PAHs)。当使用菲作为碳源进行应激代谢时,观察到 T5 细胞发生了显著变化。红外光谱分析显示,细菌表面存在 -C=C- 和 Ph-O-C(芳香族)基团,这有利于多环芳烃吸附在磷脂层上,对细胞膜造成破坏。扫描电子显微镜(SEM)分析表明细胞形态发生了变化,包括下表面出现大量皱褶和细胞膜折叠。透射电子显微镜(TEM)观察显示,营养条件充足的非应激细菌积累了更多的脂质。然而,应激组则含有更多的蛋白质。研究发现,应激代谢导致 T5 细胞的蛋白质含量增加了 16.4%,氧化还原酶的活性增加了一倍多。这些生理生化变化增强了应激细菌有效降解多环芳烃的能力,从而缩短了降解周期。这些发现为修复多环芳烃污染提供了宝贵的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of stress metabolism on physiological and biochemical reaction and polycyclic aromatic hydrocarbons degradation ability of Bacillus. sp

Effects of stress metabolism on physiological and biochemical reaction and polycyclic aromatic hydrocarbons degradation ability of Bacillus. sp

A strain of Bacillus licheniformis T5 was isolated from soil contaminated with crude oil due to its efficient degradation of polycyclic aromatic hydrocarbons (PAHs). When subjected to stress metabolism using phenanthrene as a carbon source, significant changes were observed in T5 cells. Infrared spectrum analysis revealed the presence of -C=C- and Ph-O-C (aromatic) groups on the bacterial surface, facilitating the adsorption of PAHs on the phospholipid layer and causing damage to the cell membrane. Scanning electron microscope (SEM) analysis showed the changes of cell morphology, including a large number of folds on the lower surface and the folding of cell membrane. Transmission electron microscope (TEM) observation showed that non-stressed bacteria with adequate nutritional conditions accumulated more lipids. However, the stress group contained more protein. It was found that stress metabolism led to the increase of protein content in T5 cells by 16.4% and the activity of oxidoreductase more than doubled. These physiological and biochemical changes enhance the ability of stressed bacteria to degrade PAHs efficiently, thereby reducing the degradation cycle. The findings offer valuable insights for the remediation of PAHs pollution.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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