Didrik H. Grevskott , Francisco Salvà-Serra , Edward R.B. Moore , Nachiket P. Marathe
{"title":"11873型大肠埃希菌新序列中携带一种新的CTX-M-15携带多重耐药1/2型杂交IncC质粒。","authors":"Didrik H. Grevskott , Francisco Salvà-Serra , Edward R.B. Moore , Nachiket P. Marathe","doi":"10.1016/j.jgar.2024.08.008","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15–carrying <em>Escherichia coli</em> strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the <em>E. coli</em> strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing.</div></div><div><h3>Methods</h3><div>Isolation of <em>E. coli</em> was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two <em>E. coli</em> strains (3–338 and 5–325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing.</div></div><div><h3>Results</h3><div>The complete genome of strain 3–338 (GenBank accession no. CP130007–17) was assembled into a circular chromosome of 4.65 Mb and 10 plasmids (between 2 and 148 kb). Strain 5–325 (CP130018–27) exhibited a circular chromosome of 4.7 Mb and 9 plasmids (between 2 and 148 kb). Both strains carried an identical type 1/2 hybrid IncC plasmid (∼148 kb) harbouring multiple antibiotic resistance genes (ARGs), including <em>bla</em><sub>CTX−M-15</sub>, <em>bla</em><sub>OXA-1</sub>, <em>bla</em><sub>TEM-1</sub>, <em>qnrS1, sul2, aphA1, aacC2, mph(A)</em> and <em>floR</em>. This plasmid also carried heavy metal resistance genes, such as <em>chrA</em> and <em>arsR.</em> Strain 5–325 carried an additional IncFIB plasmid (∼78 kb) harbouring additional ARGs, including <em>bla</em><sub>TEM-1</sub>, <em>qnrS1, tet</em>(A), <em>dfrA14, sul2, strA</em> and <em>strB</em>.</div></div><div><h3>Conclusions</h3><div>Our study shows the emergence of a CTX-M-15–carrying type 1/2 hybrid IncC plasmid in novel <em>E. coli</em> ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 109-113"},"PeriodicalIF":3.7000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Escherichia coli novel sequence type 11873 harbours a new CTX-M-15–carrying multidrug resistance type 1/2 hybrid IncC plasmid\",\"authors\":\"Didrik H. Grevskott , Francisco Salvà-Serra , Edward R.B. Moore , Nachiket P. Marathe\",\"doi\":\"10.1016/j.jgar.2024.08.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15–carrying <em>Escherichia coli</em> strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the <em>E. coli</em> strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing.</div></div><div><h3>Methods</h3><div>Isolation of <em>E. coli</em> was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two <em>E. coli</em> strains (3–338 and 5–325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing.</div></div><div><h3>Results</h3><div>The complete genome of strain 3–338 (GenBank accession no. CP130007–17) was assembled into a circular chromosome of 4.65 Mb and 10 plasmids (between 2 and 148 kb). Strain 5–325 (CP130018–27) exhibited a circular chromosome of 4.7 Mb and 9 plasmids (between 2 and 148 kb). Both strains carried an identical type 1/2 hybrid IncC plasmid (∼148 kb) harbouring multiple antibiotic resistance genes (ARGs), including <em>bla</em><sub>CTX−M-15</sub>, <em>bla</em><sub>OXA-1</sub>, <em>bla</em><sub>TEM-1</sub>, <em>qnrS1, sul2, aphA1, aacC2, mph(A)</em> and <em>floR</em>. This plasmid also carried heavy metal resistance genes, such as <em>chrA</em> and <em>arsR.</em> Strain 5–325 carried an additional IncFIB plasmid (∼78 kb) harbouring additional ARGs, including <em>bla</em><sub>TEM-1</sub>, <em>qnrS1, tet</em>(A), <em>dfrA14, sul2, strA</em> and <em>strB</em>.</div></div><div><h3>Conclusions</h3><div>Our study shows the emergence of a CTX-M-15–carrying type 1/2 hybrid IncC plasmid in novel <em>E. coli</em> ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors.</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"39 \",\"pages\":\"Pages 109-113\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001607\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001607","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Escherichia coli novel sequence type 11873 harbours a new CTX-M-15–carrying multidrug resistance type 1/2 hybrid IncC plasmid
Objective
The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15–carrying Escherichia coli strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the E. coli strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing.
Methods
Isolation of E. coli was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two E. coli strains (3–338 and 5–325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing.
Results
The complete genome of strain 3–338 (GenBank accession no. CP130007–17) was assembled into a circular chromosome of 4.65 Mb and 10 plasmids (between 2 and 148 kb). Strain 5–325 (CP130018–27) exhibited a circular chromosome of 4.7 Mb and 9 plasmids (between 2 and 148 kb). Both strains carried an identical type 1/2 hybrid IncC plasmid (∼148 kb) harbouring multiple antibiotic resistance genes (ARGs), including blaCTX−M-15, blaOXA-1, blaTEM-1, qnrS1, sul2, aphA1, aacC2, mph(A) and floR. This plasmid also carried heavy metal resistance genes, such as chrA and arsR. Strain 5–325 carried an additional IncFIB plasmid (∼78 kb) harbouring additional ARGs, including blaTEM-1, qnrS1, tet(A), dfrA14, sul2, strA and strB.
Conclusions
Our study shows the emergence of a CTX-M-15–carrying type 1/2 hybrid IncC plasmid in novel E. coli ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.