探索作为肝外胆管癌预后标志物的 LGR5:表达分析和临床相关性的启示。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Hisashi Tamada, Takeshi Uehara, Takahiro Yoshizawa, Mai Iwaya, Shiho Asaka, Tomoyuki Nakajima, Masato Kamakura, Hiroyoshi Ota
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引用次数: 0

摘要

背景:富亮氨酸重复含G蛋白偶联受体5(LGR5)是结直肠癌的癌症干细胞(CSC)标志物,也可能是其他癌症类型的CSC标志物。有关肝外胆管癌(ECC)中 LGR5 表达的研究很少:我们使用高灵敏度的 RNA 原位杂交技术 RNAscope 分析了 LGR5 的表达。我们从信州大学医院的医学档案中选取了 53 例 ECC,并使用组织芯片对其进行了分析。LGR5 表达水平分为表达组和无表达组。分析了 LGR5 的表达和临床病理特征:结果:在 25 例病例中,未发现 LGR5 阳性点。在 28 个病例中,在癌细胞中观察到一些 LGR5 阳性点,同时还观察到多种 LGR5 阳性细胞。在腺管形成中,LGR5表达明显。分化良好至中度分化类型的 LGR5 表达明显高于分化不良类型(p = 0.0268)。LGR5 的表达与良好的总生存期(p = 0.0219)和无病生存期(DFS)(p = 0.0228)相关。LGR5的高表达与良好分化至中度分化类型相关,表明预后良好。在DFS方面,多变量分析表明,LGR5的高表达是一个独立的有利预后因素(p = 0.0397):这些研究结果表明,LGR5是一种很有前景的新型预后标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring LGR5 as a prognostic marker of extrahepatic cholangiocarcinoma: insights from expression analysis and clinical correlations.

Background: Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) is a cancer stem cell (CSC) marker of colorectal cancer and may be a CSC marker of other cancer types. Few studies have been conducted on LGR5 expression in extrahepatic cholangiocarcinoma (ECC).

Methods: We analyzed LGR5 expression using RNAscope, a highly sensitive RNA in situ hybridization technique. Fifty-three ECCs were selected from the medical archives at Shinshu University Hospital and analyzed using a tissue microarray. LGR5 expression levels were divided into expression and no expression groups. LGR5 expression and clinicopathological characteristics were analyzed.

Results: Among 25 cases, no LGR5-positive dots were identified. Among 28 cases, some LGR5-positive dots were observed in carcinoma cells, together with a wide range of LGR5-positive cells. LGR5 expression was conspicuous in glandular duct formations. Well- to moderately differentiated types showed significantly higher LGR5 expression than the poorly differentiated type (p = 0.0268). LGR5 expression was associated with good overall survival (p = 0.0219) and good disease-free survival (DFS) (p = 0.0228). High LGR5 expression was associated with well- to moderately-differentiated types, indicating a favorable prognosis. In terms of DFS, multivariate analysis showed that high LGR5 expression was an independent favorable prognostic factor (p = 0.0397).

Conclusions: These findings suggest that LGR5 is a promising, novel prognostic marker.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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