{"title":"在多孔聚酰亚胺薄膜中孔隙选择性固定 pH 敏感聚合物和葡萄糖氧化酶以检测葡萄糖","authors":"","doi":"10.1016/j.reactfunctpolym.2024.106027","DOIUrl":null,"url":null,"abstract":"<div><p>Glucose selective oxidizing enzyme of glucose oxidase (GOD) was immobilized on the carboxyl group (–COOH) functionalized porous polyimide (PI) film (PI-GOD film). The –COOH functionalized film was fabricated by the modified breath figure method (BF) by casting the PI solution under humid conditions containing KOH. The amine group of GOD was covalently bonded to the PI-COOH film under catalytic conditions for the PI-GOD film. The PI-GOD film induced the color change of the bromothymol blue (BTB) indicator solution from green to yellow by adding glucose due to the oxidation of glucose by GOD to gluconic acid acting as an acidic source. Amine-terminated pH-sensitive polymethacrylic acid polymer (PMAA) was additionally immobilized to the PI-GOD film for the PI-GOD-PMAA film. The morphology of the pore surface of the PI-GOD-PMAA film was changed by the addition of glucose due to the coil-to-globule transition of the pH-sensitive polymer by the production of gluconic acid as a result of the reaction between glucose and GOD. The insulin adsorbed on the film was released by adding glucose due to the morphology change of the pH-sensitive polymer. The detection sensitivity of glucose was increased by the pore-selectively immobilized GOD acting as a micro-reactor.</p></div>","PeriodicalId":20916,"journal":{"name":"Reactive & Functional Polymers","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pore-selective immobilization of pH-sensitive polymer and glucose oxidase in the porous polyimide film for detection of glucose\",\"authors\":\"\",\"doi\":\"10.1016/j.reactfunctpolym.2024.106027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Glucose selective oxidizing enzyme of glucose oxidase (GOD) was immobilized on the carboxyl group (–COOH) functionalized porous polyimide (PI) film (PI-GOD film). The –COOH functionalized film was fabricated by the modified breath figure method (BF) by casting the PI solution under humid conditions containing KOH. The amine group of GOD was covalently bonded to the PI-COOH film under catalytic conditions for the PI-GOD film. The PI-GOD film induced the color change of the bromothymol blue (BTB) indicator solution from green to yellow by adding glucose due to the oxidation of glucose by GOD to gluconic acid acting as an acidic source. Amine-terminated pH-sensitive polymethacrylic acid polymer (PMAA) was additionally immobilized to the PI-GOD film for the PI-GOD-PMAA film. The morphology of the pore surface of the PI-GOD-PMAA film was changed by the addition of glucose due to the coil-to-globule transition of the pH-sensitive polymer by the production of gluconic acid as a result of the reaction between glucose and GOD. The insulin adsorbed on the film was released by adding glucose due to the morphology change of the pH-sensitive polymer. The detection sensitivity of glucose was increased by the pore-selectively immobilized GOD acting as a micro-reactor.</p></div>\",\"PeriodicalId\":20916,\"journal\":{\"name\":\"Reactive & Functional Polymers\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2024-08-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reactive & Functional Polymers\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1381514824002025\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reactive & Functional Polymers","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1381514824002025","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
摘要
葡萄糖氧化酶（GOD）的葡萄糖选择性氧化酶被固定在羧基（-COOH）功能化多孔聚酰亚胺（PI）薄膜（PI-GOD 薄膜）上。羧基官能化薄膜是通过改良呼吸图法（BF）在含有 KOH 的潮湿条件下浇铸 PI 溶液而制成的。在 PI-GOD 膜的催化条件下，GOD 的胺基团与 PI-COOH 膜共价键合。PI-GOD 薄膜通过添加葡萄糖，诱导溴百里酚蓝（BTB）指示剂溶液的颜色从绿色变为黄色，这是由于葡萄糖被 GOD 氧化成葡萄糖酸作为酸性源。在 PI-GOD 薄膜上还固定了胺封端 pH 敏感性聚甲基丙烯酸聚合物（PMAA），制成 PI-GOD-PMAA 薄膜。加入葡萄糖后，PI-GOD-PMAA 薄膜孔隙表面的形态发生了变化，这是由于葡萄糖与 GOD 反应生成葡萄糖酸，pH 值敏感聚合物发生了线圈到球状的转变。由于 pH 值敏感聚合物的形态发生变化，吸附在薄膜上的胰岛素在加入葡萄糖后被释放出来。孔隙选择性固定的 GOD 起到了微反应器的作用，从而提高了葡萄糖的检测灵敏度。
Pore-selective immobilization of pH-sensitive polymer and glucose oxidase in the porous polyimide film for detection of glucose
Glucose selective oxidizing enzyme of glucose oxidase (GOD) was immobilized on the carboxyl group (–COOH) functionalized porous polyimide (PI) film (PI-GOD film). The –COOH functionalized film was fabricated by the modified breath figure method (BF) by casting the PI solution under humid conditions containing KOH. The amine group of GOD was covalently bonded to the PI-COOH film under catalytic conditions for the PI-GOD film. The PI-GOD film induced the color change of the bromothymol blue (BTB) indicator solution from green to yellow by adding glucose due to the oxidation of glucose by GOD to gluconic acid acting as an acidic source. Amine-terminated pH-sensitive polymethacrylic acid polymer (PMAA) was additionally immobilized to the PI-GOD film for the PI-GOD-PMAA film. The morphology of the pore surface of the PI-GOD-PMAA film was changed by the addition of glucose due to the coil-to-globule transition of the pH-sensitive polymer by the production of gluconic acid as a result of the reaction between glucose and GOD. The insulin adsorbed on the film was released by adding glucose due to the morphology change of the pH-sensitive polymer. The detection sensitivity of glucose was increased by the pore-selectively immobilized GOD acting as a micro-reactor.
期刊介绍：
Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers.
Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.
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