基于纳米笼阵列的固相电化学发光酶电极用于高灵敏度检测胆固醇。

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Xinying Ma, Zhe Zhang, Yanyan Zheng, Jiyang Liu
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引用次数: 0

摘要

方便灵敏地检测代谢物对于了解人类健康状况和药物开发具有重要意义。基于酶催化反应产物过氧化氢(H2O2),固相电化学发光(ECL)酶电极在代谢物检测中显示出巨大的潜力。在此,我们利用静电纳米笼阵列制作了一种基于密闭发射器和固定化酶的固相 ECL 酶传感器,构建了一个灵敏检测胆固醇的平台。静电笼纳米通道由双极和双层垂直排列介孔二氧化硅薄膜(bp-VMSF)组成。bp-VMSF 的上层是氨基修饰的带正电的 VMSF(p-VMSF),下层是带负电的 VMSF(n-VMSF)。最常用的 ECL 探针三(联吡啶)钌(II) (Ru(bpy)32+) 通过 n-VMSF 的静电吸附和上层 p-VMSF 的静电排斥被固定在 n-VMSF 中,从而产生明显增强且稳定的 ECL 信号。扫描电子显微镜(SEM)和电化学方法对静电笼的成功制备进行了表征。用醛衍生 bp-VMSF 外表面的氨基后,共价固定了胆固醇氧化酶(ChOx)分子。循环伏安法(CV)和电化学阻抗谱(EIS)对酶电极的成功构建进行了表征。当溶液中存在相应的酶底物胆固醇时,Ru(铋)32+ 的 ECL 信号被酶催化反应产物 H2O2 所淬灭,从而实现了对胆固醇的高灵敏度检测。检测胆固醇的线性范围为 0.05 mM 至 5.0 mM,检测限为 1.5 μM。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol.

The convenient and sensitive detection of metabolites is of great significance for understanding human health status and drug development. Solid-phase electrochemiluminescence (ECL) enzyme electrodes show great potential in metabolite detection based on the enzyme-catalyzed reaction product hydrogen peroxide (H2O2). Herein, a solid-phase ECL enzyme sensor was fabricated based on a confined emitter and an immobilized enzyme using electrostatic nanocage array, constructing a platform for the sensitive detection of cholesterol. The electrostatic cage nanochannel consists of a bipolar and bilayer vertically aligned mesoporous silica film (bp-VMSF). The upper layer of bp-VMSF is an amino-modified, positively charged VMSF (p-VMSF), and the lower layer is a negatively charged VMSF (n-VMSF). The most commonly used ECL probe tris(bipyridine)ruthenium(II) (Ru(bpy)32+) is fixed in n-VMSF by electrostatic adsorption from n-VMSF and electrostatic repulsion from the upper p-VMSF, generating significantly enhanced and stable ECL signals. The successful preparation of the electrostatic cage was characterized by scanning electron microscopy (SEM) and electrochemical methods. After amino groups on the outer surface of bp-VMSF were derivatized with aldehyde, cholesterol oxidase (ChOx) molecules were covalently immobilized. The successful construction of the enzyme electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). When the corresponding enzyme substrate, cholesterol, was present in the solution, the ECL signal of Ru(bpy)32+ was quenched by the enzyme-catalyzed reaction product H2O2, enabling the high-sensitivity detection of cholesterol. The linear range for detecting cholesterol was from 0.05 mM to 5.0 mM, with a limit of detection (LOD) of 1.5 μM.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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