[弥漫大 B 细胞淋巴瘤患者 LMR 和 CD163+TAM 的预后价值］

Q4 Medicine

中国实验血液学杂志 Pub Date : 2024-08-01 DOI:10.19746/j.cnki.issn.1009-2137.2024.04.018

Xue Xu, Zong-Yuan Ye

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The number of CD163+TAM was negatively correlated with LMR (r =-0.58) and positively correlated with monocyte count (r =0.46). The cut-off values of LMR and CD163+TAM determined by ROC curve were 2.95 and 29/HPF, respectively, and based on this, the patients were divided into low LMR group and high LMR group, as well as low CD163+TAM group and high CD163+TAM group. The proportion of patients with clinical stage III-IV, IPI score 3-5 and bone marrow infiltration in the low LMR group were higher than those in the high LMR group (P < 0.05). The proportion of patients with clinical stage III-IV, IPI score 3-5, elevated LDH level and bone marrow infiltration in the high CD163+TAM group were higher than those in the low CD163+TAM group (P < 0.05). There was a positive correlation between LMR and OS (r =0.43) and a negative correlation between CD163+TAM and OS (r =-0.65). 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引用次数: 0

摘要

目的研究弥漫大B细胞淋巴瘤（DLBCL）患者淋巴细胞与单核细胞比值（LMR）和CD163+肿瘤相关巨噬细胞（TAM）的预后价值：方法：收集了63名新确诊的弥漫大B细胞淋巴瘤患者的外周血和淋巴结组织。根据血常规检查结果计算外周血中淋巴细胞和单核细胞的数量。淋巴结中 CD163+TAM 的水平通过免疫组化检测。通过 ROC 曲线确定 LMR 和 CD163+TAM 的临界值，并分析 LMR 和 CD163+TAM 在 DLBCL 患者中的预后价值：63例新诊断的DLBCL患者的LMR水平为（3.69±1.71），CD163+TAM的中位值为26/HPF。CD163+TAM 的数量与 LMR 呈负相关（r =-0.58），与单核细胞计数呈正相关（r =0.46）。根据 ROC 曲线确定的 LMR 和 CD163+TAM 临界值分别为 2.95 和 29/HPF，并据此将患者分为低 LMR 组和高 LMR 组，以及低 CD163+TAM 组和高 CD163+TAM 组。低 LMR 组临床分期 III-IV、IPI 评分 3-5 和骨髓浸润的患者比例高于高 LMR 组（P<0.05）。高CD163+TAM组临床III-IV期、IPI评分3-5分、LDH水平升高和骨髓浸润的患者比例高于低CD163+TAM组（P＜0.05）。LMR与OS呈正相关（r =0.43），CD163+TAM与OS呈负相关（r =-0.65）。低LMR和高CD163+TAM的DLBCL患者的OS较短（P < 0.05）：结论：低LMR和高CD163+TAM可作为DLBCL患者不良预后的生物学标志物。

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[Prognostic Value of LMR and CD163⁺TAM for Patients with Diffuse Large B Cell Lymphoma].

Objective: To investigate the prognostic value of lymphocyte-to-monocyte ratio (LMR) and CD163⁺tumor-associated macrophages (TAM) in patients with diffuse large B cell lymphoma (DLBCL).

Methods: Peripheral blood and lymph node tissues were collected from 63 newly diagnosed DLBCL patients. LMR was calculated by the number of lymphocytes and monocytes in peripheral blood from the result of blood routine examination. The level of CD163⁺TAM in lymph nodes was detected by immunohistochemistry. The cut-off values of LMR and CD163⁺TAM were determined by ROC curves, and the prognostic value of LMR and CD163⁺TAM in DLBCL patients was analyzed.

Results: The LMR level of 63 newly diagnosed DLBCL patients was 3.69±1.71, and the median value of CD163⁺TAM was 26/HPF. The number of CD163⁺TAM was negatively correlated with LMR (r =-0.58) and positively correlated with monocyte count (r =0.46). The cut-off values of LMR and CD163⁺TAM determined by ROC curve were 2.95 and 29/HPF, respectively, and based on this, the patients were divided into low LMR group and high LMR group, as well as low CD163⁺TAM group and high CD163⁺TAM group. The proportion of patients with clinical stage III-IV, IPI score 3-5 and bone marrow infiltration in the low LMR group were higher than those in the high LMR group (P < 0.05). The proportion of patients with clinical stage III-IV, IPI score 3-5, elevated LDH level and bone marrow infiltration in the high CD163⁺TAM group were higher than those in the low CD163⁺TAM group (P < 0.05). There was a positive correlation between LMR and OS (r =0.43) and a negative correlation between CD163⁺TAM and OS (r =-0.65). DLBCL patients with low LMR and high CD163⁺TAM had shorter OS (P < 0.05).