Myrciaria cauliflora 水乙醇提取物的植物化学成分及对金黄色葡萄球菌和鲍曼不动杆菌的抗菌和抗生物膜作用

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Luciane Dias de Oliveira, Ana Luisa Monteiro Ribeiro, Sthéfani de Oliveira Dias, Geovani Moreira da Cruz, Raquel Teles de Menezes, Lara Steffany de Carvalho, Mariana Gadelho Gimenez Diamantino, Thaís Cristine Pereira, Maria Cristina Marcucci, Amjad Abu Hasna
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引用次数: 0

摘要

金黄色葡萄球菌和鲍曼不动杆菌是机会性病原体,两者都参与不同的口腔感染。本研究旨在分析菜花菌水乙醇提取物的植物化学成分,并评估其对金黄色葡萄球菌(ATCC 6538)和鲍曼不动杆菌(ATCC 19606;多重耐药临床菌株 58004、50098、566006 和 H557)的抗菌和抗生物膜作用。制备了菜花菌水乙醇提取物,并对其中的可溶性固形物、黄酮类化合物和酚类化合物进行了定量。随后进行了高效液相色谱法(HPLC)测定。根据临床和实验室标准研究所的 M7-A6 标准,采用肉汤微稀释法测定了最低抑菌浓度,随后又测定了最低杀菌浓度。然后,分析对生物膜最有效的浓度。统计分析采用方差分析法和 Tukey 检验法。所制备的菜花藻水乙醇提取物的可溶性固形物含量为 2.22%。此外,使用槲皮素标准曲线测定的总黄酮含量为 0.040 mg/mL。此外,使用没食子酸标准曲线测定的总酚含量为 0.729 mg/mL。HPLC 分析显示出没食子酸(11.80 m)、对香豆酸(12.09 m)、肉桂酸衍生物(19.02 m)和鞣花酸(29.83 m)的峰值。萃取物对所有测试菌株都有抗菌和抗生物膜作用。然而,对所有测试细菌最有效的抗菌浓度为 5.55 毫克/毫升。因此,这些化学成分证明菜花水乙醇提取物能有效减少金黄色葡萄球菌(标准菌株)和鲍曼不动杆菌(标准菌株和临床菌株)的生物膜形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Myrciaria cauliflora Hydroethanolic Extract against Staphylococcus aureus and Acinetobacter baumannii.

Staphylococcus aureus and Acinetobacter baumannii are opportunistic pathogens, and both are involved in different oral infections. This work aimed to analyze the phytochemical composition of Myrciaria cauliflora hydroethanolic extract and to evaluate its antimicrobial and antibiofilm action against Staphylococcus aureus (ATCC 6538) and Acinetobacter baumannii (ATCC 19606; multi-resistant clinical strains 58004, 50098, 566006, and H557). Myrciaria cauliflora hydroethanolic extract was prepared, and the content of soluble solids, flavonoids, and phenols was quantified. High-performance liquid chromatography (HPLC) was performed later. The minimum inhibitory concentration was determined using the broth microdilution method according to the Clinical and Laboratory Standards Institute, standard M7-A6, and subsequently, its minimum bactericidal concentration was determined. Then, the most effective concentrations were analyzed against biofilms. Statistical analysis was performed using the ANOVA method with Tukey's test. The soluble solids content in the prepared hydroethanolic extract of M. cauliflora was 2.22%. Additionally, the total flavonoid content, measured using the quercetin standard curve, was 0.040 mg/mL. Furthermore, the total phenol content, determined using the gallic acid standard curve, was 0.729 mg/mL. HPLC analysis presented peaks of gallic acid (11.80 m), p-coumaric acid (12.09 m), cinnamic acid derivative (19.02 m), and ellagic acid (29.83 m). The extract demonstrated antimicrobial and antibiofilm action against all tested strains. However, the most effective antibacterial concentration against all the tested bacteria was 5.55 mg/mL. Therefore, these chemical components justify that M. cauliflora hydroethanolic extract is effective in reducing biofilm formation in S. aureus (standard strain) and A. baumannii (standard and clinical strains).

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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