Alline Teixeira Valeriano, Lais Santos Camara, Vanessa de Fátima Bernardes, Fabiano Sviatopolk-Mirsky Pais, Flávio Marcos Gomes Araújo, Anna Christina de Matos Salim, Gabriel da Rocha Fernandes, Fernanda Stussi, Carolina Cavalieri Gomes, Pedro Paulo de Andrade Santos, Lélia Batista de Souza, Ricardo Santiago Gomez, Marina Gonçalves Diniz
{"title":"MMP13的表达和活性表明其在釉母细胞瘤骨吸收中的作用","authors":"Alline Teixeira Valeriano, Lais Santos Camara, Vanessa de Fátima Bernardes, Fabiano Sviatopolk-Mirsky Pais, Flávio Marcos Gomes Araújo, Anna Christina de Matos Salim, Gabriel da Rocha Fernandes, Fernanda Stussi, Carolina Cavalieri Gomes, Pedro Paulo de Andrade Santos, Lélia Batista de Souza, Ricardo Santiago Gomez, Marina Gonçalves Diniz","doi":"10.1111/jop.13577","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Ameloblastoma is a locally destructive benign odontogenic tumor. While the neoplastic cells of conventional ameloblastoma can infiltrate the connective tissue and bone, in unicystic ameloblastoma the epithelium is encapsulated. The mechanisms driving ameloblastoma's bone resorption remains unclear.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>RNA sequencing (RNA-seq) was performed in a discovery cohort of conventional ameloblastoma, and pathway enrichment analysis was carried out. mRNA levels of <i>MMP13</i>, a gene associated with bone resorption, were assessed using RT-qPCR in a larger cohort of conventional ameloblastoma and in unicystic ameloblastoma. Zymogram gels and the immunoexpression profile of collagenase 3 (encoded by <i>MMP13</i> gene) were evaluated as well.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Enriched pathways related to bone mineralization and upregulation of <i>MMP13</i> were observed in ameloblastomas. Collagenolytic activity of collagenase 3 was detected in the tumor lysates. Collagenase 3 immunopositivity was observed in ameloblastomatous epithelium infiltrating the fibrous capsule of unicystic ameloblastoma. At the tumor–bone interface, collagenase 3 expression was detected in stromal cells, osteoblasts, and osteocytes.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The results indicate a potential involvement of <i>MMP13</i> in ameloblastoma-related bone resorption and progression.</p>\n </section>\n </div>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":"53 9","pages":"577-583"},"PeriodicalIF":2.7000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"MMP13 Expression and Activity Suggest Its Role in Bone Resorption in Ameloblastomas\",\"authors\":\"Alline Teixeira Valeriano, Lais Santos Camara, Vanessa de Fátima Bernardes, Fabiano Sviatopolk-Mirsky Pais, Flávio Marcos Gomes Araújo, Anna Christina de Matos Salim, Gabriel da Rocha Fernandes, Fernanda Stussi, Carolina Cavalieri Gomes, Pedro Paulo de Andrade Santos, Lélia Batista de Souza, Ricardo Santiago Gomez, Marina Gonçalves Diniz\",\"doi\":\"10.1111/jop.13577\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Ameloblastoma is a locally destructive benign odontogenic tumor. While the neoplastic cells of conventional ameloblastoma can infiltrate the connective tissue and bone, in unicystic ameloblastoma the epithelium is encapsulated. The mechanisms driving ameloblastoma's bone resorption remains unclear.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>RNA sequencing (RNA-seq) was performed in a discovery cohort of conventional ameloblastoma, and pathway enrichment analysis was carried out. mRNA levels of <i>MMP13</i>, a gene associated with bone resorption, were assessed using RT-qPCR in a larger cohort of conventional ameloblastoma and in unicystic ameloblastoma. Zymogram gels and the immunoexpression profile of collagenase 3 (encoded by <i>MMP13</i> gene) were evaluated as well.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Enriched pathways related to bone mineralization and upregulation of <i>MMP13</i> were observed in ameloblastomas. Collagenolytic activity of collagenase 3 was detected in the tumor lysates. Collagenase 3 immunopositivity was observed in ameloblastomatous epithelium infiltrating the fibrous capsule of unicystic ameloblastoma. 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MMP13 Expression and Activity Suggest Its Role in Bone Resorption in Ameloblastomas
Background
Ameloblastoma is a locally destructive benign odontogenic tumor. While the neoplastic cells of conventional ameloblastoma can infiltrate the connective tissue and bone, in unicystic ameloblastoma the epithelium is encapsulated. The mechanisms driving ameloblastoma's bone resorption remains unclear.
Methods
RNA sequencing (RNA-seq) was performed in a discovery cohort of conventional ameloblastoma, and pathway enrichment analysis was carried out. mRNA levels of MMP13, a gene associated with bone resorption, were assessed using RT-qPCR in a larger cohort of conventional ameloblastoma and in unicystic ameloblastoma. Zymogram gels and the immunoexpression profile of collagenase 3 (encoded by MMP13 gene) were evaluated as well.
Results
Enriched pathways related to bone mineralization and upregulation of MMP13 were observed in ameloblastomas. Collagenolytic activity of collagenase 3 was detected in the tumor lysates. Collagenase 3 immunopositivity was observed in ameloblastomatous epithelium infiltrating the fibrous capsule of unicystic ameloblastoma. At the tumor–bone interface, collagenase 3 expression was detected in stromal cells, osteoblasts, and osteocytes.
Conclusion
The results indicate a potential involvement of MMP13 in ameloblastoma-related bone resorption and progression.
期刊介绍：
The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.
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