纤连蛋白功能化:增强藻酸盐/羟基磷灰石支架上细胞动态培养的一种方法

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL
Bianca Zumbo, Benedetta Guagnini, Barbara Medagli, Davide Porrelli, Gianluca Turco
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引用次数: 0

摘要

骨缺损是全球关注的健康问题;骨组织工程(BTE)是最有希望降低患者发病率并克服自体移植和异体移植骨固有缺点的替代方法。三维支架具有提供结构支撑和模拟天然骨微环境的潜力,因此在这一领域举足轻重。按照已公布的方案,经过凝胶化步骤和冷冻干燥步骤,制备出了由海藻酸盐和羟基磷灰石组成的三维多孔结构。尽管海藻酸盐经常被用于组织再生,但这种多糖的生物惰性阻碍了细胞的正常定植和增殖。因此,这项工作的目的是利用纤连蛋白促进细胞与生物材料之间的相互作用和粘附,从而增强生物特性。这种细胞外基质蛋白被物理吸附在支架上,并用环境扫描电子显微镜(eSEM)和微二喹啉酸(μBCA)蛋白检测法评估了它的存在。MG-63 细胞系被用于支架上的静态和动态(即在生物反应器中)三维细胞培养。使用生物反应器可以更好地交换养分和氧气,更好地清除构建体内部的细胞代谢产物,从而模拟生理环境。与非功能化支架相比,功能化支架改善了细胞的增殖和定植;在动态培养条件下,添加纤连蛋白的效果更加明显,细胞明显粘附在功能化支架表面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fibronectin Functionalization: A Way to Enhance Dynamic Cell Culture on Alginate/Hydroxyapatite Scaffolds.

Bone defects are a global health concern; bone tissue engineering (BTE) is the most promising alternative to reduce patient morbidity and overcome the inherent drawbacks of autograft and allograft bone. Three-dimensional scaffolds are pivotal in this field due to their potential to provide structural support and mimic the natural bone microenvironment. Following an already published protocol, a 3D porous structure consisting of alginate and hydroxyapatite was prepared after a gelation step and a freezing-drying step. Despite the frequent use of alginate in tissue regeneration, the biological inertness of this polysaccharide hampers proper cell colonization and proliferation. Therefore, the purpose of this work was to enhance the biological properties by promoting the interaction and adhesion between cells and biomaterial with the use of Fibronectin. This extracellular matrix protein was physically adsorbed on the scaffold, and its presence was evaluated with environmental scanning electron microscopy (eSEM) and the Micro-Bicinchoninic Acid (μBCA) protein assay. The MG-63 cell line was used for both static and dynamic (i.e., in bioreactor) 3D cell culturing on the scaffolds. The use of the bioreactor allowed for a better exchange of nutrients and oxygen and a better removal of cell catabolites from the inner portion of the construct, mimicking the physiological environment. The functionalized scaffolds showed an improvement in cell proliferation and colonization compared to non-functionalized ones; the effect of the addition of Fibronectin was more evident in the dynamic culturing conditions, where the cells clearly adhered on the surface of functionalized scaffolds.

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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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