Lesten Eliez Chisomo Chatepa, Bonface Mwamatope, Ibrahim Chikowe, Kingsley George Masamba
{"title":"溶剂萃取对马拉维两种药用植物叶提取物的植物成分和体外抗氧化活性特性的影响。","authors":"Lesten Eliez Chisomo Chatepa, Bonface Mwamatope, Ibrahim Chikowe, Kingsley George Masamba","doi":"10.1186/s12906-024-04619-7","DOIUrl":null,"url":null,"abstract":"<p><p>This study evaluated and compared the phytochemical and antioxidant properties of the solvent extracts of Azadirachta indica A. Juss and Vernonia amygdalina Del leaves. Methanolic and aqueous extracts showed high (P ≤ 0.05) extract yields (in %), compared to chloroform and ethyl acetate extracts from both V. amygdalina and A. indica leaves. The study exhibited high phytochemical content in methanol and aqueous extracts compared to chloroform and ethyl acetate extracts, confirming the potential for medicinal use. V. amygdalina methanol and aqueous extracts had higher (P ≤ 0.05) total phenolic content (TPC), in mg GAE/gDW, (158.810±0.846 and 217.883±0.265, respectively) than chloroform (37.574±0.118) and ethyl acetate (104.758±0.236) but higher ethyl acetate content in A. indica extracts. Low polar solvents extracted high (P ≤ 0.05) total flavonoids, in mgQE/gDW, (367.051±0.858 and 149.808±0.009) compared to high polar solvents (14.863±0.071 and 54.226±0.014 ) in V. amygdalina while as in A. indica leaf extracts, low polar solvents showed high TFC ( 658.469±3.451 and 275.288±10.490) compared to high polar solvents (26.312±0.063 and 48.858±0.063) respectively. In vitro total antioxidant capacity, in mg/g, was higher in polar solvents than in low-polar solvents, ranging from 34.300±1.784 to 121.015±6.839 for A. indica ethyl acetate and methanolic extracts. A strong correlation between TPC and tannic acid content was observed, except in A. indica methanolic extracts of A. indica. Ferric reducing power was high, except for V. amygdalina chloroform and methanol leaf extracts, which were lower (P≤ 0.05) than that of the standard ascorbic acid. The study revealed that high polar solvents, such as methanol and water, are more efficient in the extraction of antioxidants from A. indica but lower in V. amygdalina extracts. 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Juss and Vernonia amygdalina Del leaves. Methanolic and aqueous extracts showed high (P ≤ 0.05) extract yields (in %), compared to chloroform and ethyl acetate extracts from both V. amygdalina and A. indica leaves. The study exhibited high phytochemical content in methanol and aqueous extracts compared to chloroform and ethyl acetate extracts, confirming the potential for medicinal use. V. amygdalina methanol and aqueous extracts had higher (P ≤ 0.05) total phenolic content (TPC), in mg GAE/gDW, (158.810±0.846 and 217.883±0.265, respectively) than chloroform (37.574±0.118) and ethyl acetate (104.758±0.236) but higher ethyl acetate content in A. indica extracts. Low polar solvents extracted high (P ≤ 0.05) total flavonoids, in mgQE/gDW, (367.051±0.858 and 149.808±0.009) compared to high polar solvents (14.863±0.071 and 54.226±0.014 ) in V. amygdalina while as in A. indica leaf extracts, low polar solvents showed high TFC ( 658.469±3.451 and 275.288±10.490) compared to high polar solvents (26.312±0.063 and 48.858±0.063) respectively. In vitro total antioxidant capacity, in mg/g, was higher in polar solvents than in low-polar solvents, ranging from 34.300±1.784 to 121.015±6.839 for A. indica ethyl acetate and methanolic extracts. A strong correlation between TPC and tannic acid content was observed, except in A. indica methanolic extracts of A. indica. Ferric reducing power was high, except for V. amygdalina chloroform and methanol leaf extracts, which were lower (P≤ 0.05) than that of the standard ascorbic acid. The study revealed that high polar solvents, such as methanol and water, are more efficient in the extraction of antioxidants from A. indica but lower in V. amygdalina extracts. 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引用次数: 0
摘要
本研究评估并比较了 Azadirachta indica A. Juss 和 Vernonia amygdalina Del 叶子溶剂提取物的植物化学和抗氧化特性。与氯仿和乙酸乙酯提取物相比，甲醇提取物和水提取物的提取率较高（P ≤ 0.05）。研究表明，与氯仿和乙酸乙酯提取物相比，甲醇和水提取物中的植物化学成分含量较高，证实了其药用潜力。与氯仿（37.574±0.118）和乙酸乙酯（104.758±0.236）相比，V. amygdalina 甲醇和水提取物的总酚含量（TPC）（以 mg GAE/gDW 计）较高（P ≤ 0.05）（分别为 158.810±0.846 和 217.883±0.265），但在 A. indica 提取物中乙酸乙酯含量较高。与高极性溶剂（14.863±0.071 和 54.226±0.014）相比，低极性溶剂萃取的总黄酮含量较高（P ≤ 0.05），以 mgQE/gDW 计（367.051±0.858 和 149.808±0.009）。与高极性溶剂（26.312±0.063 和 48.858±0.063）相比，低极性溶剂显示出较高的 TFC（658.469±3.451 和 275.288±10.490）。体外总抗氧化能力（以毫克/克计）在极性溶剂中高于低极性溶剂，籼稻乙酸乙酯提取物和甲醇提取物的体外总抗氧化能力在 34.300±1.784 到 121.015±6.839 之间。除 A. indica 的甲醇提取物外，TPC 与单宁酸含量之间存在很强的相关性。除了 V. amygdalina 的氯仿和甲醇叶提取物的铁还原力低于标准抗坏血酸（P≤ 0.05）外，其他叶提取物的铁还原力都很高。研究结果表明，甲醇和水等高极性溶剂对籼稻抗氧化剂的提取效率较高，但对杏树叶提取物的提取效率较低。高植物化学成分含量和抗氧化能力对治疗人类的各种疾病具有重要意义。
Effects of solvent extraction on the phytoconstituents and in vitro antioxidant activity properties of leaf extracts of the two selected medicinal plants from Malawi.
This study evaluated and compared the phytochemical and antioxidant properties of the solvent extracts of Azadirachta indica A. Juss and Vernonia amygdalina Del leaves. Methanolic and aqueous extracts showed high (P ≤ 0.05) extract yields (in %), compared to chloroform and ethyl acetate extracts from both V. amygdalina and A. indica leaves. The study exhibited high phytochemical content in methanol and aqueous extracts compared to chloroform and ethyl acetate extracts, confirming the potential for medicinal use. V. amygdalina methanol and aqueous extracts had higher (P ≤ 0.05) total phenolic content (TPC), in mg GAE/gDW, (158.810±0.846 and 217.883±0.265, respectively) than chloroform (37.574±0.118) and ethyl acetate (104.758±0.236) but higher ethyl acetate content in A. indica extracts. Low polar solvents extracted high (P ≤ 0.05) total flavonoids, in mgQE/gDW, (367.051±0.858 and 149.808±0.009) compared to high polar solvents (14.863±0.071 and 54.226±0.014 ) in V. amygdalina while as in A. indica leaf extracts, low polar solvents showed high TFC ( 658.469±3.451 and 275.288±10.490) compared to high polar solvents (26.312±0.063 and 48.858±0.063) respectively. In vitro total antioxidant capacity, in mg/g, was higher in polar solvents than in low-polar solvents, ranging from 34.300±1.784 to 121.015±6.839 for A. indica ethyl acetate and methanolic extracts. A strong correlation between TPC and tannic acid content was observed, except in A. indica methanolic extracts of A. indica. Ferric reducing power was high, except for V. amygdalina chloroform and methanol leaf extracts, which were lower (P≤ 0.05) than that of the standard ascorbic acid. The study revealed that high polar solvents, such as methanol and water, are more efficient in the extraction of antioxidants from A. indica but lower in V. amygdalina extracts. High phytochemical content and antioxidative capacity could be significant in treating various diseases in humans.
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