伊朗沙勒科德(Shahrekord)大型牲畜生肉中空肠弯曲菌和大肠弯曲菌的分离、特征和抗菌谱。

Q3 Veterinary
E Rahimi, S B Mousavinafchi, A Shakerian
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引用次数: 0

摘要

弯曲杆菌属是导致全球微生物性肠炎的最常见原因之一。本研究旨在了解弯曲杆菌在伊朗大型牲畜生肉中的常见程度,并确定它们对抗生素的敏感性。研究人员从研究地区的屠宰场、肉店和餐馆收集了 550 份新鲜即食肉类样本。样本分别来自牛(138 个)、山羊(102 个)、骆驼(56 个)和绵羊(254 个)。使用普通细菌学方法和聚合酶链式反应 (PCR) 分离和鉴定弯曲杆菌属。利用聚合酶链反应进行基因分型,以确定毒力基因。采用盘扩散技术确定抗生素敏感性。在检测的 550 个肉类样本中,有 84 个(15.27%)发现了两种弯曲杆菌属。牛和骆驼样品中弯曲杆菌属的检出率分别最高(52.38%)和最低(3.57%)。在生肉中分离出的弯曲菌中,空肠弯曲菌和大肠弯曲菌占 82.14%(n=69)。在 39.28% 的样本(样本数=33)中发现空肠弯曲菌,在 42.85% 的样本(样本数=36)中发现大肠弯曲菌。其他弯曲杆菌属占样本的 17.85%(n=15)。从不同类型的大型动物样本中采集到的空肠弯曲菌中最常见的基因型是 ciaB(100%)和 flaA(100%)。另一方面,virbll(7.69%)是在不同大型动物样本中发现的发病率最低的空肠大肠杆菌菌株。大肠杆菌中最常见的基因型是 ciaB(100%)和 flaA(100%)。大肠杆菌分离株 dnaJ(0%)、wlaN(0%)、virbll(0%)和 ceuE(0%)在多个大型牲畜样本中的检出率最低。从不同样本类型和来源中分离出的弯曲杆菌属对 aphA-3-1 和 GM10 的敏感性为 100%。据报道,这些分离物对 E15(76.93%)、cmeB(69.24%)、aadE1(69.24%)、CIP5(69.24%)和 AM10(69.24%)具有抗药性。根据这项研究,在工厂化养殖的食物中发现了弯曲杆菌。因此,食用生肉或未煮熟的肉类可传播该疾病。因此,正确处理和准备肉类食品,以及从屠宰场到零售商的卫生措施,对预防弯曲杆菌感染至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation, characterization, and antimicrobial resistance profiles of Campylobacter jejuni and Campylobacter coli from raw meat of large livestock in Shahrekord, Iran.

Campylobacter spp. genera is one of the most common causes of microbial enteritis worldwide. This study aimed to find out how common Campylobacter organisms were in raw meat from large livestock in Iran, as well as to determine their antibiotic susceptibility profiles. Several 550 fresh, ready-to-eat meat samples were collected from slaughterhouses, butcher shops, and restaurants in the study region. The samples were collected from cattle (n=138), goats (n=102), camels (n=56), and sheep (n=254). Campylobacter spp. were isolated and identified using normal bacteriological methods and polymerase chain reaction (PCR). Genotyping was performed using PCR to identify virulence genes. The disc diffusion technique was used to determine antibiotic susceptibility. The two Campylobacter spp. were found in 84 (15.27%) of the 550 meat samples tested. Cattle and camel samples accounted for the highest (52.38%) and lowest (3.57%) frequencies of Campylobacter spp., respectively. There were significant differences in the prevalence of Campylobacter spp. in cattle (2=43.04 or OR=7.68, CI=3.40-17.30, P<0.01). Campylobacter jejuni and Campylobacter coli accounted for 82.14% (n=69) of Campylobacter spp. isolated from raw meat. While C. jejuni was found in 39.28% of the samples (n=33), C. coli was observed in 42.85% (n=36). Other Campylobacter spp. formed 17.85 % (n=15) of the samples. The most common genotypes observed in C. jejuni bacteria collected from different types of large animal samples were ciaB (100%) and flaA (100%). On the other hand, virbll (7.69%) was the C. jejuni strain found with the lowest incidence in different large animal samples. The most frequent genotypes found in C. coli bacteria were ciaB (100%) and flaA (100%). C. coli isolates dnaJ (0%), wlaN (0%), virbll (0%), and ceuE (0%) were detected with the lowest frequency in several samples from large livestock. Campylobacter spp. isolated from different sample types and sources were 100% sensitive to aphA-3-1 and GM10. The isolates were reported to be resistant to E15 (76.93%), cmeB (69.24%), aadE1 (69.24%), CIP5 (69.24%), and AM10 (69.24%). According to this study, Campylobacter was found in food from factory farming. Consequently, the disease can be transmitted by eating raw or undercooked meat. Therefore, proper handling and preparation of meat meals, as well as hygiene measures from the slaughterhouse to the retailer, are critical in preventing Campylobacter infections.

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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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