在大鼠实验性膜性肾病模型中,T 滤泡辅助细胞的扩张与疾病进展有关。

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Li Deng, Bishun Deng, Ziling Zhao, Huijie Huang, Xiaowan Wang, Ruimin Tian, Guohua Li, Enyu Liang, Anping Peng, Peifeng Ke, Peng Xu, Min He
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引用次数: 0

摘要

背景:T滤泡辅助细胞(Tfh)通过促进B细胞反应驱动体液免疫,但Tfh细胞在特发性膜性肾病(IMN)发病机制中的功能作用仍不清楚:本研究旨在建立大鼠实验性膜性肾病模型,研究Tfh细胞的表型特征,并分析Tfh细胞之间具有临床意义的相关性:用抗Fx1A血清免疫Sprague Dawley大鼠,诱导被动性Heymann肾炎(PHN)大鼠。用流式细胞术(FC)分析 Tfh 和 B 细胞亚群的频率。评估了血清中白细胞介素-21(IL-21)的浓度、脾脏单核细胞(MNCs)中 IL-21 和 B 细胞淋巴瘤 6(Bcl-6)的相对 mRNA 表达水平以及肾脏中 CD4+ T 细胞和 IL-21 的浸润情况。分析了这些指标之间的潜在相关性:结果:与对照组相比,PHN 大鼠脾脏中 Tfh 细胞、诱导性 T 细胞协同刺激因子阳性(ICOS+)Tfh 细胞的百分比和 Bcl-6 的 mRNA 表达均明显增加。血清和肾脏中检测到 IL-21 表达升高。值得注意的是,PHN 大鼠脾脏 ICOS+ Tfh 细胞的百分比与 24 小时尿蛋白浓度呈正相关(r = 0.676,p = 0.011):这些数据表明,ICOS+ Tfh 细胞有助于 IMN 的发展,它们可能是 IMN 的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expansion of T follicular helper cells is associated with disease progression in rat experimental membranous nephropathy model.

Background: T follicular helper (Tfh) cells drive humoral immunity by facilitating B cell responses, but the functional role of Tfh cells in the pathogenesis of idiopathic membranous nephropathy (IMN) remains unclear.

Objectives: This study aimed to establish a rat experimental membranous nephropathy model, investigate the phenotypic characteristics of Tfh cells, and analyze a clinically significant correlation between Tfh cells.

Material and methods: Passive Heymann nephritis (PHN) rats were induced by immunizing Sprague Dawley rats with anti-Fx1A serum. The frequency of Tfh and B cell subsets was analyzed with flow cytometry (FC). The serum concentration of interleukin-21 (IL-21), the relative mRNA expression levels of IL-21 and B cell lymphoma 6 (Bcl-6) in spleen mononuclear cells (MNCs), and the kidney infiltration of CD4+ T cells and IL-21 were assessed. The potential correlations among these measures were analyzed.

Results: In comparison with the control group, significantly increased percentages of Tfh cells, inducible T cell co-stimulator-positive (ICOS+) Tfh cells, and mRNA expression of Bcl-6 were detected in the spleen of PHN rats. Elevated IL-21 expression was detected in the serum and kidneys. Remarkably, the percentage of splenic ICOS+ Tfh cells was positively correlated with 24 h urine protein concentrations (r = 0.676, p = 0.011) in PHN rats.

Conclusion: These data indicate that ICOS+ Tfh cells contribute to development of IMN, and they might be potential therapeutic targets for IMN.

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来源期刊
Advances in Clinical and Experimental Medicine
Advances in Clinical and Experimental Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
3.70
自引率
4.80%
发文量
153
审稿时长
6-12 weeks
期刊介绍: Advances in Clinical and Experimental Medicine has been published by the Wroclaw Medical University since 1992. Establishing the medical journal was the idea of Prof. Bogumił Halawa, Chair of the Department of Cardiology, and was fully supported by the Rector of Wroclaw Medical University, Prof. Zbigniew Knapik. Prof. Halawa was also the first editor-in-chief, between 1992-1997. The journal, then entitled "Postępy Medycyny Klinicznej i Doświadczalnej", appeared quarterly. Prof. Leszek Paradowski was editor-in-chief from 1997-1999. In 1998 he initiated alterations in the profile and cover design of the journal which were accepted by the Editorial Board. The title was changed to Advances in Clinical and Experimental Medicine. Articles in English were welcomed. A number of outstanding representatives of medical science from Poland and abroad were invited to participate in the newly established International Editorial Staff. Prof. Antonina Harłozińska-Szmyrka was editor-in-chief in years 2000-2005, in years 2006-2007 once again prof. Leszek Paradowski and prof. Maria Podolak-Dawidziak was editor-in-chief in years 2008-2016. Since 2017 the editor-in chief is prof. Maciej Bagłaj. Since July 2005, original papers have been published only in English. Case reports are no longer accepted. The manuscripts are reviewed by two independent reviewers and a statistical reviewer, and English texts are proofread by a native speaker. The journal has been indexed in several databases: Scopus, Ulrich’sTM International Periodicals Directory, Index Copernicus and since 2007 in Thomson Reuters databases: Science Citation Index Expanded i Journal Citation Reports/Science Edition. In 2010 the journal obtained Impact Factor which is now 1.179 pts. Articles published in the journal are worth 15 points among Polish journals according to the Polish Committee for Scientific Research and 169.43 points according to the Index Copernicus. Since November 7, 2012, Advances in Clinical and Experimental Medicine has been indexed and included in National Library of Medicine’s MEDLINE database. English abstracts printed in the journal are included and searchable using PubMed http://www.ncbi.nlm.nih.gov/pubmed.
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