有氧运动激活 AMPK/PGC-1α 通路,抑制心肌细胞凋亡,改善线粒体和梗死心脏功能

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qiu Shen, Xinyue Wu, Chuan Huang, Xinyu Ding, Chunxiao Wan
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引用次数: 0

摘要

近年来,有氧运动(AE)作为一种非侵入性的治疗工具吸引了大量研究人员的关注。越来越多的证据表明,有氧运动对多种疾病具有保护作用。在本研究中,我们旨在确定有氧运动是否能抑制梗死心肌细胞的凋亡并保护心脏。对心肌梗塞后(post-MI)小鼠服用 AE 可改善其心脏和身体功能。心肌梗塞后小鼠心肌组织的透射电子显微镜和腺苷-5'-三磷酸(ATP)测定结果显示,心肌梗塞后小鼠的线粒体数量增加,但ATP含量下降。值得注意的是,AE 能明显逆转这种变化。免疫荧光/TUNEL 染色检测结果显示,AE 可抑制心肌细胞凋亡。通过对心肌组织进行免疫印迹,我们发现 AE 提高了抗凋亡蛋白 Bcl-2/Bax 的水平,显著降低了促凋亡蛋白 caspase-3 的表达,并激活了 AMPK/PGC-1α 信号通路。我们的研究结果证明,AE 能激活 AMPK/PGC-1α 信号通路,提高线粒体供能能力,有效抑制心肌细胞凋亡。因此,AE 可被视为一种很有前景的梗死后治疗干预方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Aerobic Exercise Activates AMPK/PGC-1α Pathway, Inhibits Cardiomyocyte Apoptosis Improves Mitochondrial and Infarcted Heart Function

Aerobic Exercise Activates AMPK/PGC-1α Pathway, Inhibits Cardiomyocyte Apoptosis Improves Mitochondrial and Infarcted Heart Function

Aerobic Exercise Activates AMPK/PGC-1α Pathway, Inhibits Cardiomyocyte Apoptosis Improves Mitochondrial and Infarcted Heart Function

Aerobic exercise (AE) has attracted considerable research attention as a non-invasive therapeutic tool in recent years. Accumulating evidence has revealed its protective role against a wide range of diseases. In this study, we aimed to establish whether AE could inhibit apoptosis in infarcted cardiomyocytes and protect the heart. AE in post-myocardial infarction (post-MI) mice improved their cardiac and physical functions. Transmission electron microscopy of myocardial tissue and adenosine 5'-triphosphate (ATP) assay findings revealed an increased mitochondrial number but decreased ATP content in the post-MI mice. Notably, this change was significantly reversed by AE. Immunofluorescence/ TUNEL staining assay results showed that AE inhibited cardiomyocyte apoptosis. Using immunoblotting of myocardial tissues, we found that AE increased the level of the anti-apoptotic protein Bcl-2/Bax, significantly decreased the expression of the pro-apoptotic protein caspase-3, and activated the AMPK/PGC-1α signaling pathway. Our findings provide evidence that AE activates the AMPK/PGC-1α signaling pathway, improves mitochondrial energy supply capacity, and effectively inhibits apoptosis in cardiomyocytes. Therefore, AE can be considered a promising post-infarction therapeutic intervention.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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