用于蛋白质分离的聚酰胺胺树枝状聚合物改性聚偏氟乙烯微孔膜

IF 4.5 3区 工程技术 Q1 CHEMISTRY, APPLIED
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引用次数: 0

摘要

压力驱动过滤膜的分离效率主要由膜孔径决定。在分离小分子时,孔径较大的膜通常通量较高,但排斥率较低或为零。在蛋白质分离中,孔径小于目标蛋白质分子尺寸的超滤(UF)膜通常用于尺寸抑制。受纳滤(NF)膜分离机制的启发,我们假设在适当孔径的膜中引入带电基团,可以显著增强膜电荷与蛋白质电荷之间的电相互作用。当蛋白质分子接近或通过带电的纳米级膜通道时,在纳米级距离上发生的这种增强作用可使大大小于孔径的蛋白质被剔除。使用孔径相对较大的膜可能会导致通量增加。为了验证这一假设,我们对具有合适孔径的聚偏二氟乙烯(PVDF)膜进行了改性实验,使用聚酰胺胺(PAMAM)树枝状聚合物为膜引入负电荷。研究了 PVDF 膜和改性膜在分离乳清蛋白时的性能。为了评估立体阻碍和电阻碍对溶质分离的贡献,使用聚氧化乙烯(PEO)和聚丙烯酸(PAA)进行了过滤实验。使用衰减全反射-傅立叶变换红外(ATR-FTIR)、X 射线光电子能谱(XPS)和扫描电子显微镜(SEM)等技术对膜进行了表征。结果表明,改性提高了乳清蛋白的截留效率。PVDF 膜的乳清蛋白截留率和渗透通量分别为 58.9% 和 15.3 LMH。经碱性处理或 PAMAM-G3.5 树枝状聚合物改性后,乳清蛋白排斥率分别提高到 97.3% 和 98.8%。然而,碱性处理和 PAMAM-G3.5 树枝状聚合物修饰导致渗透通量分别降至 5.6 LMH 和 2.3 LMH。这表明,增加膜电荷可有效提高过滤膜在带电大分子分离中的分离能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Polyamidoamine dendrimer-modified polyvinylidene fluoride microporous membranes for protein separation

Polyamidoamine dendrimer-modified polyvinylidene fluoride microporous membranes for protein separation

The separation efficiency of pressure-driven filtration membranes is primarily dictated by the membrane pore size. Membranes with larger pores typically demonstrate high flux but low or zero rejection when it comes to separating small molecules. In protein separation, ultrafiltration (UF) membranes with pore sizes smaller than the molecular dimensions of target proteins are commonly used for size rejection. Taking inspiration from the separation mechanism of nanofiltration (NF) membranes, we hypothesize that introducing charged groups into membranes of appropriate pore sizes could significantly enhance the electrical interaction between membrane charges and protein charges. This enhancement, occurring at the nanoscale distance when protein molecules approach or pass through charged nanoscale membrane channels, may enable the rejection of proteins substantially smaller than the pore size. Using membranes with relatively large pore sizes could lead to an increase in flux. To test this hypothesis, we conducted experiments involving the modification of polyvinylidene fluoride (PVDF) membranes with suitable pore sizes, using polyamidoamine (PAMAM) dendrimers to introduce negative charges to the membranes. The performance of the PVDF membranes and the modified membranes were investigated in the separation of whey proteins. To evaluate the contribution of steric and electrical hindrance to the solute separation, filtration experiments were performed using polyethylene oxide (PEO) and polyacrylic acid (PAA). The membranes were characterized using techniques such as attenuated total reflectance-Fourier transform infrared (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). The results indicate that the modification enhances the rejection efficiency of whey proteins. The whey protein rejection and permeate flux for PVDF membranes were 58.9% and 15.3 LMH, respectively. Following alkaline treatment or PAMAM-G3.5 dendrimer modification, the whey protein rejection increased to 97.3% and 98.8%, respectively. However, alkaline treatment and PAMAM-G3.5 dendrimer modification resulted in a reduction of permeate flux to 5.6 LMH and 2.3 LMH, respectively. This suggests that increasing membrane charge effectively enhances the separation ability of filtration membranes in charged macromolecule separation.

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来源期刊
Reactive & Functional Polymers
Reactive & Functional Polymers 工程技术-高分子科学
CiteScore
8.90
自引率
5.90%
发文量
259
审稿时长
27 days
期刊介绍: Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers. Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.
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