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引用次数: 0
摘要
目的：囊状棘球蚴病（CE）是一种被忽视的热带疾病，在全球流行，尤其是在农村地区。以往的研究评估了肝包虫病患者的免疫反应，但没有研究同时评估肺包虫病患者的 Th1、Th2 和 Th17 水平。本研究旨在通过流式细胞术分析填补这一文献空白：方法：从健康对照组（HC）志愿者和活动性肺CE囊肿患者的血样中分离出外周血单核细胞（PBMC）。分别使用干扰素（IFN）-γ、白细胞介素（IL）-4 和 IL-17 抗体分析外周血单核细胞，以评估 CD3 + CD4 + T 细胞群中 Th1、Th2 和 Th17 细胞的水平：结果：我们的分析表明，CE 患者的 Th2 水平升高，而 Th1 和 Th17 细胞计数在 HC 志愿者和肺 CE 患者之间没有明显差异：结论：研究结果表明，Th1/Th2/Th17 细胞在肺癌发病机制中的调节失衡。建议今后的研究对肺癌和肝癌的免疫反应进行比较，以证实这些发现，并评估与两种临床形式的肺癌相关的免疫病理的任何潜在差异。
Evaluation of Th1/Th2/Th17 Balance in Pulmonary Cystic Echinococcosis Patients
Purpose
Cystic echinococcosis (CE) is a neglected tropical disease prevalent worldwide, particularly in rural areas. Previous studies evaluated immune responses in patients with hepatic CE, however none had assessed Th1, Th2 and Th17 levels simultaneously in pulmonary CE patients. This study aimed to fill this gap in literature by using flow cytometry analysis.
Methods
Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy control (HC) volunteers and patients with active pulmonary CE cysts. The PBMCs were analysed to evaluate Th1, Th2, and Th17 cell levels within the CD3 + CD4 + T-cell population, using antibodies against interferon (IFN)-γ, interleukin (IL)-4, and IL-17, respectively.
Results
Our analysis revealed elevated Th2 levels in CE patients, while Th1 and Th17 cell counts showed no significant difference between HC volunteers and patients with pulmonary CE.
Conclusion
The results indicate an imbalanced Th1/Th2/Th17 cell regulation in the pathogenesis of pulmonary CE. Future studies are recommended to compare immune responses between pulmonary and hepatic CE to confirm these findings and evaluate any potential difference in the immunopathology associated with the two clinical forms of CE.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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