Samaneh Mazaherifar, Saiedeh Erfanian, Kavous Solhjoo, Abazar Roustazadeh, Mohammad Darayesh, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Seyede Manizhe Heidarnejadi, Sima Rasti, Amir Abdoli
{"title":"在伊朗四个利什曼病流行地区的大利什曼病临床分离株中检测利什曼病 RNA 病毒 2 (LRV2)。","authors":"Samaneh Mazaherifar, Saiedeh Erfanian, Kavous Solhjoo, Abazar Roustazadeh, Mohammad Darayesh, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Seyede Manizhe Heidarnejadi, Sima Rasti, Amir Abdoli","doi":"10.1007/s11686-024-00915-x","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p><i>Leishmania</i> RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of <i>Leishmania</i> parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the <i>Leishmania major</i> isolates in four endemic regions of Iran.</p><h3>Methods</h3><p>In a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of <i>L. major</i> from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and <i>kmp</i> genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.</p><h3>Results</h3><p>LRV2 was detected in 27.6% (50 out of 181) of <i>L. major</i> isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.</p><h3>Conclusion</h3><p>This study confirms the presence of LRV2 in clinical isolates of <i>L. major</i> from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"69 4","pages":"2046 - 2050"},"PeriodicalIF":1.2000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran\",\"authors\":\"Samaneh Mazaherifar, Saiedeh Erfanian, Kavous Solhjoo, Abazar Roustazadeh, Mohammad Darayesh, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Seyede Manizhe Heidarnejadi, Sima Rasti, Amir Abdoli\",\"doi\":\"10.1007/s11686-024-00915-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p><i>Leishmania</i> RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of <i>Leishmania</i> parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the <i>Leishmania major</i> isolates in four endemic regions of Iran.</p><h3>Methods</h3><p>In a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of <i>L. major</i> from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and <i>kmp</i> genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.</p><h3>Results</h3><p>LRV2 was detected in 27.6% (50 out of 181) of <i>L. major</i> isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.</p><h3>Conclusion</h3><p>This study confirms the presence of LRV2 in clinical isolates of <i>L. major</i> from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"69 4\",\"pages\":\"2046 - 2050\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-024-00915-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-024-00915-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran
Purpose
Leishmania RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of Leishmania parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the Leishmania major isolates in four endemic regions of Iran.
Methods
In a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of L. major from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and kmp genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.
Results
LRV2 was detected in 27.6% (50 out of 181) of L. major isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.
Conclusion
This study confirms the presence of LRV2 in clinical isolates of L. major from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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