Sushma Krishnan, Snehal Dilip Karpe, Hithesh Kumar, Lucy B Nongbri, Vignesh Venkateswaran, Ramanathan Sowdhamini, Ewald Grosse-Wilde, Bill S Hansson, Renee M Borges
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引用次数: 0
摘要
昆虫的一个基本适应策略是进化出嗅觉受体(OR)来识别重要的挥发性环境化学线索。我们的模式物种 Ceratosolen fusciceps 是一种专门为无花果授粉的黄蜂,它可能拥有一种嗅觉受体,使其能够在高度多变的环境中分辨出无花果特有的挥发性物质。利用新组装的基因组指导转录组,我们注释了该物种的 63 个 ORs,并结合其他膜翅目物种重建了 Ceratosolen ORs 的系统发育。表达分析表明,虽然ORs主要在雌性触角中表达,但也有20%在非触角组织中表达,如头部、胸部、腹部、腿部、翅膀和卵巢。在头部的 OR30C 和翅膀的 OR60C 中观察到了特定的上调表达。我们在雌性栉水母的所有主要身体部位都发现了 OR 的表达,这表明 OR 在整个身体中发挥着新的作用。在印度南部(城市)和东北部(农村)这两个地理位置相距甚远的地区进一步研究姬蛙的 OR 表达,发现不同地区的 OR 表达水平各不相同。这种差异可能与观察到的无花果挥发物在这些地区之间的差异相似,并为昆虫 ORs 的进化及其在不同地理位置和组织中的表达提供了新的见解。
Sensing volatiles throughout the body: geographic- and tissue-specific olfactory receptor expression in the fig wasp.
An essential adaptive strategy in insects is the evolution of olfactory receptors (ORs) to recognize important volatile environmental chemical cues. Our model species, Ceratosolen fusciceps, a specialist wasp pollinator of Ficus racemosa, likely possesses an OR repertoire that allows it to distinguish fig-specific volatiles in highly variable environments. Using a newly assembled genome-guided transcriptome, we annotated 63 ORs in the species and reconstructed the phylogeny of Ceratosolen ORs in conjunction with other hymenopteran species. Expression analysis showed that though ORs were mainly expressed in the female antennae, 20% were also expressed in nonantennal tissues such as the head, thorax, abdomen, legs, wings, and ovipositor. Specific upregulated expression was observed in OR30C in the head and OR60C in the wings. We identified OR expression from all major body parts of female C. fusciceps, suggesting novel roles of ORs throughout the body. Further examination of the OR expression of C. fusciceps in widely separated geographical locations, that is, South (urban) and Northeast (rural) India, revealed distinct OR expression levels in different locations. This discrepancy likely parallels the observed variation in fig volatiles between these regions and provides new insights into the evolution of insect ORs and their expression across geographical locations and tissues.
期刊介绍:
Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.