{"title":"不同分期食管鳞癌中TAMs和TILs浸润的空间异质性及其预后意义","authors":"Ya Li, Jia Liu, Liwen Qi, Xin Yuan, Kaige Yang, Yilin Ren, Qi Shi, Guixuan Xu, Weinan Wang, Chenghua Luo, Lianghai Wang, Weihua Liang, Zengtao He, Wenhu Zhou, Jing Fei, Weigang Chen, Wenyi Gu, Feng Li, Jianming Hu","doi":"10.1016/j.dld.2024.08.003","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The prognostic value and clinical relevance of tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) in esophageal squamous cell carcinoma (ESCC) remain unclear.</p><p><strong>Aims: </strong>To investigate the prognostic value and functional involvement of TILs in ESCC.</p><p><strong>Methods: </strong>We included 40 patients across different stages of ESCC from Xinjiang. Multiplex fluorescent immunohistochemistry characterized TILs and TAMs. TILs in different tumor regions were quantified and correlated with overall survival (OS) using log-rank test and Cox regression analyses.</p><p><strong>Results: </strong>Invasive ESCC exhibited increased CD4 T cells and Tregs compared to carcinoma in situ, with a higher Tregs/CD4 T cells ratio (p < 0.05). TAMs, primarily in stromal regions, were significantly associated with Foxp3+ cells (p < 0.05). Higher infiltration of stromal TAMs and a higher CD4/CD8 T cells ratio correlated with poorer OS, while a higher CD8 T/Foxp3+ cells ratio indicated better survival. Multivariate Cox analysis revealed TNM stage, tumor length, and stromal CD4/CD8 T cells ratio as independent prognostic factors (p < 0.05). An immune prognostic risk score-based nomogram was constructed to predict patient outcomes.</p><p><strong>Conclusions: </strong>The spatial distribution and abundance of TILs significantly correlated with prognosis, providing a useful immune classification for ESCC.</p>","PeriodicalId":11268,"journal":{"name":"Digestive and Liver Disease","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spatial heterogeneity and prognostic significance of TAMs and TILs infiltrates in different staging esophageal squamous carcinoma.\",\"authors\":\"Ya Li, Jia Liu, Liwen Qi, Xin Yuan, Kaige Yang, Yilin Ren, Qi Shi, Guixuan Xu, Weinan Wang, Chenghua Luo, Lianghai Wang, Weihua Liang, Zengtao He, Wenhu Zhou, Jing Fei, Weigang Chen, Wenyi Gu, Feng Li, Jianming Hu\",\"doi\":\"10.1016/j.dld.2024.08.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The prognostic value and clinical relevance of tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) in esophageal squamous cell carcinoma (ESCC) remain unclear.</p><p><strong>Aims: </strong>To investigate the prognostic value and functional involvement of TILs in ESCC.</p><p><strong>Methods: </strong>We included 40 patients across different stages of ESCC from Xinjiang. Multiplex fluorescent immunohistochemistry characterized TILs and TAMs. TILs in different tumor regions were quantified and correlated with overall survival (OS) using log-rank test and Cox regression analyses.</p><p><strong>Results: </strong>Invasive ESCC exhibited increased CD4 T cells and Tregs compared to carcinoma in situ, with a higher Tregs/CD4 T cells ratio (p < 0.05). TAMs, primarily in stromal regions, were significantly associated with Foxp3+ cells (p < 0.05). Higher infiltration of stromal TAMs and a higher CD4/CD8 T cells ratio correlated with poorer OS, while a higher CD8 T/Foxp3+ cells ratio indicated better survival. Multivariate Cox analysis revealed TNM stage, tumor length, and stromal CD4/CD8 T cells ratio as independent prognostic factors (p < 0.05). An immune prognostic risk score-based nomogram was constructed to predict patient outcomes.</p><p><strong>Conclusions: </strong>The spatial distribution and abundance of TILs significantly correlated with prognosis, providing a useful immune classification for ESCC.</p>\",\"PeriodicalId\":11268,\"journal\":{\"name\":\"Digestive and Liver Disease\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Digestive and Liver Disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.dld.2024.08.003\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Digestive and Liver Disease","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.dld.2024.08.003","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：肿瘤浸润淋巴细胞（TILs）和肿瘤相关巨噬细胞（TAMs）在食管鳞状细胞癌（ESCC）中的预后价值和临床意义仍不明确：方法：纳入新疆40例不同分期的ESCC患者。多重荧光免疫组化鉴定 TILs 和 TAMs。采用对数秩检验和 Cox 回归分析对不同肿瘤区域的 TILs 进行量化，并将其与总生存率（OS）相关联：结果：与原位癌相比，浸润性 ESCC 表现出更多的 CD4 T 细胞和 Tregs，Tregs/CD4 T 细胞比值更高（p < 0.05）。主要位于基质区的TAMs与Foxp3+细胞明显相关（p < 0.05）。较高的基质TAM浸润和较高的CD4/CD8 T细胞比值与较差的OS相关，而较高的CD8 T/Foxp3+细胞比值则表明生存率较高。多变量 Cox 分析显示，TNM 分期、肿瘤长度和基质 CD4/CD8 T 细胞比值是独立的预后因素（P < 0.05）。基于免疫预后风险评分的提名图被用来预测患者的预后：结论：TILs的空间分布和丰度与预后密切相关，为ESCC提供了有用的免疫分类方法。
Spatial heterogeneity and prognostic significance of TAMs and TILs infiltrates in different staging esophageal squamous carcinoma.
Background: The prognostic value and clinical relevance of tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) in esophageal squamous cell carcinoma (ESCC) remain unclear.
Aims: To investigate the prognostic value and functional involvement of TILs in ESCC.
Methods: We included 40 patients across different stages of ESCC from Xinjiang. Multiplex fluorescent immunohistochemistry characterized TILs and TAMs. TILs in different tumor regions were quantified and correlated with overall survival (OS) using log-rank test and Cox regression analyses.
Results: Invasive ESCC exhibited increased CD4 T cells and Tregs compared to carcinoma in situ, with a higher Tregs/CD4 T cells ratio (p < 0.05). TAMs, primarily in stromal regions, were significantly associated with Foxp3+ cells (p < 0.05). Higher infiltration of stromal TAMs and a higher CD4/CD8 T cells ratio correlated with poorer OS, while a higher CD8 T/Foxp3+ cells ratio indicated better survival. Multivariate Cox analysis revealed TNM stage, tumor length, and stromal CD4/CD8 T cells ratio as independent prognostic factors (p < 0.05). An immune prognostic risk score-based nomogram was constructed to predict patient outcomes.
Conclusions: The spatial distribution and abundance of TILs significantly correlated with prognosis, providing a useful immune classification for ESCC.
期刊介绍：
Digestive and Liver Disease is an international journal of Gastroenterology and Hepatology. It is the official journal of Italian Association for the Study of the Liver (AISF); Italian Association for the Study of the Pancreas (AISP); Italian Association for Digestive Endoscopy (SIED); Italian Association for Hospital Gastroenterologists and Digestive Endoscopists (AIGO); Italian Society of Gastroenterology (SIGE); Italian Society of Pediatric Gastroenterology and Hepatology (SIGENP) and Italian Group for the Study of Inflammatory Bowel Disease (IG-IBD).
Digestive and Liver Disease publishes papers on basic and clinical research in the field of gastroenterology and hepatology.
Contributions consist of:
Original Papers
Correspondence to the Editor
Editorials, Reviews and Special Articles
Progress Reports
Image of the Month
Congress Proceedings
Symposia and Mini-symposia.
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