{"title":"具有更强催化活性的共轭微孔聚合物-支架酶级联系统","authors":"Zhenhua Wu , Jiafu Shi , Boyu Zhang , Yushuai Jiao , Xiangxuan Meng , Ziyi Chu , Yu Chen , Yiran Cheng , Zhongyi Jiang","doi":"10.1016/S1872-2067(24)60088-4","DOIUrl":null,"url":null,"abstract":"<div><p>Enhancing catalytic activity of multi-enzyme <em>in vitro</em> through substrate channeling effect is promising yet challenging. Herein, conjugated microporous polymers (CMPs)-scaffolded integrated enzyme cascade systems (I-ECSs) are constructed through co-entrapping glucose oxidase (GOx) and horseradish peroxidase (HRP), in which hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is the intermediate product. The interplay of low-resistance mass transfer pathway and appropriate pore wall-H<sub>2</sub>O<sub>2</sub> interactions facilitates the directed transfer of H<sub>2</sub>O<sub>2</sub>, resulting in 2.4-fold and 5.0-fold elevation in catalytic activity compared to free ECSs and separated ECSs, respectively. The substrate channeling effect could be regulated by altering the mass ratio of GOx to HRP. Besides, I-ECSs demonstrate excellent stabilities in harsh environments and multiple recycling.</p></div>","PeriodicalId":9832,"journal":{"name":"Chinese Journal of Catalysis","volume":"63 ","pages":"Pages 213-223"},"PeriodicalIF":15.7000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Conjugated microporous polymers-scaffolded enzyme cascade systems with enhanced catalytic activity\",\"authors\":\"Zhenhua Wu , Jiafu Shi , Boyu Zhang , Yushuai Jiao , Xiangxuan Meng , Ziyi Chu , Yu Chen , Yiran Cheng , Zhongyi Jiang\",\"doi\":\"10.1016/S1872-2067(24)60088-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Enhancing catalytic activity of multi-enzyme <em>in vitro</em> through substrate channeling effect is promising yet challenging. Herein, conjugated microporous polymers (CMPs)-scaffolded integrated enzyme cascade systems (I-ECSs) are constructed through co-entrapping glucose oxidase (GOx) and horseradish peroxidase (HRP), in which hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is the intermediate product. The interplay of low-resistance mass transfer pathway and appropriate pore wall-H<sub>2</sub>O<sub>2</sub> interactions facilitates the directed transfer of H<sub>2</sub>O<sub>2</sub>, resulting in 2.4-fold and 5.0-fold elevation in catalytic activity compared to free ECSs and separated ECSs, respectively. The substrate channeling effect could be regulated by altering the mass ratio of GOx to HRP. Besides, I-ECSs demonstrate excellent stabilities in harsh environments and multiple recycling.</p></div>\",\"PeriodicalId\":9832,\"journal\":{\"name\":\"Chinese Journal of Catalysis\",\"volume\":\"63 \",\"pages\":\"Pages 213-223\"},\"PeriodicalIF\":15.7000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Catalysis\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1872206724600884\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Catalysis","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1872206724600884","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Conjugated microporous polymers-scaffolded enzyme cascade systems with enhanced catalytic activity
Enhancing catalytic activity of multi-enzyme in vitro through substrate channeling effect is promising yet challenging. Herein, conjugated microporous polymers (CMPs)-scaffolded integrated enzyme cascade systems (I-ECSs) are constructed through co-entrapping glucose oxidase (GOx) and horseradish peroxidase (HRP), in which hydrogen peroxide (H2O2) is the intermediate product. The interplay of low-resistance mass transfer pathway and appropriate pore wall-H2O2 interactions facilitates the directed transfer of H2O2, resulting in 2.4-fold and 5.0-fold elevation in catalytic activity compared to free ECSs and separated ECSs, respectively. The substrate channeling effect could be regulated by altering the mass ratio of GOx to HRP. Besides, I-ECSs demonstrate excellent stabilities in harsh environments and multiple recycling.
期刊介绍：
The journal covers a broad scope, encompassing new trends in catalysis for applications in energy production, environmental protection, and the preparation of materials, petroleum chemicals, and fine chemicals. It explores the scientific foundation for preparing and activating catalysts of commercial interest, emphasizing representative models.The focus includes spectroscopic methods for structural characterization, especially in situ techniques, as well as new theoretical methods with practical impact in catalysis and catalytic reactions.The journal delves into the relationship between homogeneous and heterogeneous catalysis and includes theoretical studies on the structure and reactivity of catalysts.Additionally, contributions on photocatalysis, biocatalysis, surface science, and catalysis-related chemical kinetics are welcomed.
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