拮抗海马 CA1 区的两种奥曲肽受体可降低束缚应激引起的抗痛觉效应

IF 2.5 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Elaheh Danesh , Shahin Hassanpour , Bita Vazir , Mohammad Saghafi , Mohadeseh Ghalandari-Shamami , Abbas Haghparast
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引用次数: 0

摘要

研究表明,压力相关症状会导致荷尔蒙和神经发生变化,影响痛阈和痛觉行为。奥曲肽受体(OX1r和OX2r)在应激诱导镇痛(SIA)中的确切作用仍有待全面阐明。目前的研究旨在评估在大鼠模型中使用针对 OX1r 和 OX2r 的拮抗剂后,急性固定束缚应激对疼痛相关行为反应的影响。大鼠在CA1区接受立体定向手术后五到七天,记录每只动物的基线弹尾潜伏期(TFL)。随后,通过植入插管给大鼠单侧注射不同剂量的 OX1r 拮抗剂(SB334867;1、3、10 和 30 nmol)、OX2r 拮抗剂(TCS OX2 29;1、3、10 和 30 nmol)或载体(0.5 μl 含有 12% DMSO 的溶液)。间隔 5 分钟后,对动物进行持续 3 小时的束缚应激(RS)。应激暴露后进行弹尾试验,每隔 60 分钟评估一次 TFL。该研究结果表明,RS能在弹尾试验中引起抗痛觉反应。向CA1显微注射OX1r和OX2r拮抗剂可减轻RS在尾叩试验中诱导的镇痛。此外,研究结果还强调了 OX2 受体在调节 SIA 中的重要作用。总之,位于海马CA1区的奥曲肽系统可能在一定程度上导致了急性疼痛背景下的SIA表现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The restraint stress-induced antinociceptive effects decreased by antagonism of both orexin receptors within the CA1 region of the hippocampus

Studies have indicated that stress-related symptoms can lead to hormonal and neural changes, affecting the pain threshold and nociceptive behaviors. The precise role of orexin receptors (OX1r and OX2r) in stress-induced analgesia (SIA) remains an inquiry yet to be comprehensively elucidated. The current investigation aimed to assess the impact of acute immobilization restraint stress on pain-related behavioral responses after administering antagonists targeting OX1r and OX2r in a rat model using the tail-flick test. After a period of five to seven days post-stereotaxic surgery in CA1, the baseline tail-flick latency (TFL) was recorded for each animal. Subsequently, rats were unilaterally administered varying doses of the OX1r antagonist (SB334867; 1, 3, 10, and 30 nmol), the OX2r antagonist (TCS OX2 29; 1, 3, 10, and 30 nmol), or a vehicle (0.5 μl solution containing 12% DMSO) through an implanted cannula. Following a 5-min interval, the animals were subjected to a restraint stress (RS) lasting for 3 h. The tail-flick test was conducted after the stress exposure, and the TFLs were assessed at 60-min intervals. The findings of this study revealed that RS elicits antinociceptive responses in the tail-flick test. Microinjection of OX1r and OX2r antagonists into the CA1 attenuated RS-induced analgesia during the tail-flick test. Furthermore, the results underscored the preeminent role of OX2 receptors in modulating SIA. In conclusion, the orexin system localized within the hippocampal CA1 region may, in part, contribute to the manifestation of SIA in the context of acute pain.

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来源期刊
Neuropeptides
Neuropeptides 医学-内分泌学与代谢
CiteScore
5.40
自引率
6.90%
发文量
55
审稿时长
>12 weeks
期刊介绍: The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.
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