Ilse Verburg , Lucia Hernández Leal , Karola Waar , John W.A. Rossen , Heike Schmitt , Silvia García-Cobos
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Whole-genome sequencing was performed of 185 <em>K. pneumoniae</em> isolates collected from hospital, nursing home, and community wastewater, the receiving wastewater treatment plant (WWTP), and clinical isolates from the investigated hospital. <em>K. pneumoniae</em> isolates from different sources were not genetically related, except for WWTP influent (46.5%) and effluent (62.5%), revealing survival of bacteria from wastewater treatment. The content of antibiotic resistance (ARGs), virulence, and plasmid replicon genes differed between <em>K. pneumoniae</em> subspecies and their origin. While chromosomal <em>bla</em> genes were specific for each <em>K. pneumoniae</em> subspecies, <em>bla</em> genes predicted in plasmid contigs were found in several <em>K. pneumoniae</em> subspecies, implying possible gene transfer between subspecies. Transferable ARGs were most abundant in patients and hospital isolates (70%), but the average number of plasmid replicon genes per isolate was similar across all sources, showing plasmid content being more relevant than plasmid quantity. 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引用次数: 0
摘要
肺炎克雷伯菌在医院内感染和抗生素耐药性传播中扮演着重要角色,因此对公共卫生构成了重大威胁。在这项研究中,我们调查了废水途径在致病菌传播中的作用,更具体地说,是在耐抗生素肺炎克雷伯菌亚种传播中的作用。我们对从医院、疗养院和社区废水、接收废水的污水处理厂(WWTP)中收集的 185 株肺炎克雷伯菌分离物以及调查医院的临床分离物进行了全基因组测序。除污水处理厂进水(46.5%)和出水(62.5%)外,不同来源的肺炎克雷伯菌分离物在基因上没有关联,这表明细菌在污水处理过程中得以存活。肺炎克雷伯菌亚种及其来源的抗生素耐药性(ARGs)、毒力和质粒复制子基因的含量各不相同。虽然染色体上的 bla 基因是每个肺炎克氏菌亚种所特有的,但质粒等位基因组中预测的 bla 基因在多个肺炎克氏菌亚种中都有发现,这意味着亚种之间可能存在基因转移。可转移的 ARGs 在患者和医院分离物中最多(70%),但所有来源的分离物中每个分离物的质粒复制子基因平均数量相似,这表明质粒含量比质粒数量更重要。大多数患者(90%)和医院废水(34%)分离物都是肺炎克氏菌肺炎亚种,只有在该亚种中才发现了yersiniabactin簇基因ybt、fyuA和irp12,以及IncFII(pECLA)、IncHI2A和IncHI2质粒复制子基因,这表明这类质粒来源于临床。
Klebsiella pneumoniae species complex: From wastewater to the environment
Klebsiella pneumoniae plays a significant role in nosocomial infections and spreading antibiotic resistance, and therefore forms a major threat to public health. In this study, we investigated the role of the wastewater pathway in the spread of pathogenic bacteria and more specifically, in the spread of antibiotic resistant Klebsiella pneumoniae subspecies. Whole-genome sequencing was performed of 185 K. pneumoniae isolates collected from hospital, nursing home, and community wastewater, the receiving wastewater treatment plant (WWTP), and clinical isolates from the investigated hospital. K. pneumoniae isolates from different sources were not genetically related, except for WWTP influent (46.5%) and effluent (62.5%), revealing survival of bacteria from wastewater treatment. The content of antibiotic resistance (ARGs), virulence, and plasmid replicon genes differed between K. pneumoniae subspecies and their origin. While chromosomal bla genes were specific for each K. pneumoniae subspecies, bla genes predicted in plasmid contigs were found in several K. pneumoniae subspecies, implying possible gene transfer between subspecies. Transferable ARGs were most abundant in patients and hospital isolates (70%), but the average number of plasmid replicon genes per isolate was similar across all sources, showing plasmid content being more relevant than plasmid quantity. Most patient (90%) and hospital wastewater (34%) isolates were K. pneumoniae subsp. pneumoniae, and the yersiniabactin cluster genes ybt, fyuA, and irp12 were only found in this subspecies, as were the IncFII(pECLA), IncHI2A, and IncHI2 plasmid replicon genes, suggesting the clinical origin of these type of plasmids.
期刊介绍:
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