免疫比容能否用作猪疫苗接种和感染研究的监测工具?

IF 3 2区 农林科学 Q1 VETERINARY SCIENCES
Mònica Sagrera, Marina Sibila, Núria Martínez-Boixaderas, Anna Maria Llorens, David Espigares, Josep Pastor, Laura Garza-Moreno, Joaquim Segalés
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引用次数: 0

摘要

背景:免疫比容是一种成本效益高且简单的技术,传统上用于评估新生仔猪的被动免疫转移。然而,该技术以前并未用于监测疫苗接种和/或感染的效果。因此,本研究以猪圆环病毒 2(PCV-2)为病原体模型,旨在评估免疫比容技术作为免疫监测工具在疫苗接种和挑战情况下的实用性。对接种过 PCV-2 疫苗(V)和未接种过 PCV-2 疫苗(NV)的 3 周龄仔猪(研究第 0 天,SD0)的免疫球蛋白比率进行监测,随后在 SD21 天对其进行病毒挑战,并随访至 SD42。还采用了其他技术(PCV-2 IgG ELISA、光学折光测定法和蛋白质图)来进一步确定免疫球蛋白分析结果的特征:结果:免疫比容、γ-球蛋白浓度和 PCV-2 S/P 值的变化趋势相似:接种 PCV-2 疫苗后下降,而接种试验性 PCV-2 后上升。然而,只有在 PCV-2 ELISA 中才发现 V 型动物和 NV 型动物之间存在显著的统计学差异。在这种情况下,V 型动物的血清中 PCV-2 酶联免疫吸附试验结果明显高于 NV 型动物:免疫比容技术代表了血清蛋白总量的一部分,与本研究中测量的所有辅助技术的相关性较低到中等。在不同时间点测定免疫比容无法监测疫苗接种和/或以 PCV-2 为病原体模型的感染效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Can immunocrit be used as a monitoring tool for swine vaccination and infection studies?

Background: The immunocrit is a cost-effective and straightforward technique traditionally used to assess passive immunity transfer to newborn piglets. However, it has not been previously used for monitoring the effect of vaccination and/or infections. Therefore, this study aimed to evaluate the usefulness of the immunocrit technique as an immunological monitoring tool in a vaccination and challenge scenario, using porcine circovirus 2 (PCV-2) as pathogen model. The immunocrit ratio was monitored in PCV-2 vaccinated (V) and non-vaccinated (NV) 3-week-old piglets (study day 0, SD0) that were subsequently challenged with this virus at SD21 and followed up to SD42. Additional techniques (PCV-2 IgG ELISA, optical refractometry, and proteinogram) were performed to further characterize the results of the immunocrit analysis.

Results: Immunocrit, γ-globulin concentration and PCV-2 S/P values followed similar dynamics: descending after PCV-2 vaccination but ascending after an experimental PCV-2 inoculation. However, statistically significant differences between V and NV animals were only found with the PCV-2 ELISA. In this case, V animals had significantly higher (p < 0.05) S/P values (S/P ratio = 0.74) than NV (S/P ratio = 0.39) pigs only after challenge at SD42. On the other hand, serum total protein obtained by refractometer (STPr) were maintained from SD0 to SD21 and increased in both groups from SD21 to SD42. Correlations between techniques were low to moderate, being the most robust ones found between immunocrit and optical refractometry (ρ = 0.41) and immunocrit with γ-globulins (ρ = 0.39). In a subset of sera, the proteinogram technique was applied to the whole serum and the supernatant of the immunocrit, with the objective to characterize indirectly the immunocrit fraction. The latter one included all protein types detectable through the proteinogram, with percentages varying between 64.3% (γ-globulins) and 82% (β-globulins).

Conclusion: The immunocrit technique represented a fraction of the total serum proteins, with low to moderate correlation with all the complementary techniques measured in this study. Its determination at different time points did not allow monitoring the effect of vaccination and/or infection using PCV-2 as a pathogen model.

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来源期刊
Porcine Health Management
Porcine Health Management Veterinary-Food Animals
CiteScore
5.40
自引率
5.90%
发文量
49
审稿时长
14 weeks
期刊介绍: Porcine Health Management (PHM) is an open access peer-reviewed journal that aims to publish relevant, novel and revised information regarding all aspects of swine health medicine and production.
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